Bulletin of Experimental Biology and Medicine

, Volume 141, Issue 4, pp 524–529

Expression of regulatory T-lymphocyte phenotype in human fetal hemopoietic and lymphoid cell culture

  • N. I. Sharova
  • A. D. Donetskova
  • I. V. Dubrovina
  • G. T. Sukhikh
  • A. A. Yarilin
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

DOI: 10.1007/s10517-006-0213-1

Cite this article as:
Sharova, N.I., Donetskova, A.D., Dubrovina, I.V. et al. Bull Exp Biol Med (2006) 141: 524. doi:10.1007/s10517-006-0213-1

Abstract

Cells with regulatory T-cell phenotype (Treg, CD4+CD25hi) were not detected in human fetal thymus, liver, bone marrow, spleen, and among blood mononuclears of 14–28-week gestation. The cells of the majority of these fetal thymuses express Treg specific marker (FOXP3 transcription marker) gene. Culturing of fetal liver and bone marrow cells on a monolayer of thymic epithelial cells induced expression of FOXP3 gene, but induction of CD4+CD25+ membrane phenotype was detected in only 1 of 8 studied cultures (in liver and bone marrow cells). Induction of Treg differentiation is to a greater extent determined by the characteristics of hemopoietic organ cells than of thymic epithelial cells.

Key Words

regulatory T cellsthymic epitheliumfetal hemopoietic organs

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • N. I. Sharova
    • 1
  • A. D. Donetskova
    • 1
  • I. V. Dubrovina
    • 2
  • G. T. Sukhikh
    • 2
  • A. A. Yarilin
    • 1
  1. 1.Institute of ImmunologyFMBARussia
  2. 2.National Center of Obstetrics, Gynecology, and PerinatologyRussian Academy of Medical SciencesMoscow