, Volume 141, Issue 4, pp 524-529

Expression of regulatory T-lymphocyte phenotype in human fetal hemopoietic and lymphoid cell culture

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Abstract

Cells with regulatory T-cell phenotype (Treg, CD4+CD25hi) were not detected in human fetal thymus, liver, bone marrow, spleen, and among blood mononuclears of 14–28-week gestation. The cells of the majority of these fetal thymuses express Treg specific marker (FOXP3 transcription marker) gene. Culturing of fetal liver and bone marrow cells on a monolayer of thymic epithelial cells induced expression of FOXP3 gene, but induction of CD4+CD25+ membrane phenotype was detected in only 1 of 8 studied cultures (in liver and bone marrow cells). Induction of Treg differentiation is to a greater extent determined by the characteristics of hemopoietic organ cells than of thymic epithelial cells.

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Translated from Kletochnye Tekhnologii v Biologii i Medicine, No. 2, pp. 96–101, April, 2006