Aquaculture International

, Volume 17, Issue 4, pp 303–315

Effects of feeding regime and probionts on the diverting microbial communities in rotifer Brachionus culture

  • Zizhong Qi
  • Kristof Dierckens
  • Tom Defoirdt
  • Patrick Sorgeloos
  • Nico Boon
  • Zhenmin Bao
  • Peter Bossier
Article

DOI: 10.1007/s10499-008-9202-x

Cite this article as:
Qi, Z., Dierckens, K., Defoirdt, T. et al. Aquacult Int (2009) 17: 303. doi:10.1007/s10499-008-9202-x

Abstract

Rotifer growth performance and microbial community changes associated with rotifer cultures were monitored while different feed types (Nannochloropsis oculata paste and the commercial yeast based feed CS-3000), different regimes (daily changes, changes per batch and no changes) and mixtures of three probionts (Phenylobacterium sp.; Gluconobacter sp. and Paracoccus denitrificans) were provided. It was shown that the dominant bacterial species in the cultures receiving either N. oculata or CS-3000 were different. However, in cultures receiving both feeds (either switching between feeds on a daily basis or on a batch basis), a high similarity in microbial community fingerprint was found. The presence of probionts was detected by the end of four batch culture cycles in spite of strong shifts of the bacterial community. By group discriminant analysis, it was found that Phenylobacterium sp. and Paracoccus sp. contributed positively to the CS-3000-fed group, while Gluconobacter sp. contributed positively to the N. oculata-fed group, although they did not appear as very dominant species.

Keywords

DGGE Feeding regime Microbial community Probiont Rotifer 

Abbreviations

CS-3000

Culture Selco 3000®

DGGE

Denaturing gradient gel electrophoresis

MC

Microbial community

MA

Marine agar

ANOVA

Analysis of variance

NSS

Nine salt solution

FASW

Filtered, autoclaved natural seawater

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Zizhong Qi
    • 1
    • 2
  • Kristof Dierckens
    • 2
  • Tom Defoirdt
    • 2
    • 3
  • Patrick Sorgeloos
    • 2
  • Nico Boon
    • 3
  • Zhenmin Bao
    • 1
  • Peter Bossier
    • 2
  1. 1.College of Marine Life SciencesOcean University of ChinaQingdaoChina
  2. 2.Laboratory of Aquaculture & Artemia Reference CenterGhent UniversityGentBelgium
  3. 3.Laboratory of Microbial Ecology and Technology (LabMET)Ghent UniversityGentBelgium

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