Original Paper

Apoptosis

, Volume 16, Issue 12, pp 1195-1204

Fas/FasL pathway-mediated alveolar macrophage apoptosis involved in human silicosis

  • San-qiao YaoAffiliated withDivision of Pneumoconiosis, School of Public Health, China Medical UniversityDepartment of Occupational and Environmental Health, School of Public Health, Hebei United UniversityKey Lab for Coal Mine Health and Safety of Hebei Province
  • , Liying Wang RojanasakulAffiliated withPathology and Physiology Research Branch, National Institute for Occupational Safety and Health (NIOSH)
  • , Zhi-yuan ChenAffiliated withDepartment of Pneumoconiosis, Beidaihe Sanitarium for China Coal Miners
  • , Ying-jun XuAffiliated withDepartment of Occupational and Environmental Health, School of Public Health, Hebei United UniversityKey Lab for Coal Mine Health and Safety of Hebei Province
  • , Yu-ping BaiAffiliated withDepartment of Occupational and Environmental Health, School of Public Health, Hebei United UniversityKey Lab for Coal Mine Health and Safety of Hebei Province
  • , Gang ChenAffiliated withDepartment of Pneumoconiosis, Beidaihe Sanitarium for China Coal Miners
  • , Xi-ying ZhangAffiliated withDepartment of Occupational and Environmental Health, School of Public Health, Hebei United University
  • , Chun-min ZhangAffiliated withDepartment of Occupational and Environmental Health, School of Public Health, Hebei United UniversityKey Lab for Coal Mine Health and Safety of Hebei Province
  • , Yan-qin YuAffiliated withDepartment of Occupational and Environmental Health, School of Public Health, Hebei United University
    • , Fu-hai ShenAffiliated withDepartment of Occupational and Environmental Health, School of Public Health, Hebei United UniversityKey Lab for Coal Mine Health and Safety of Hebei Province
    • , Ju-xiang YuanAffiliated withDepartment of Occupational and Environmental Health, School of Public Health, Hebei United UniversityKey Lab for Coal Mine Health and Safety of Hebei Province
    • , Jie ChenAffiliated withDivision of Pneumoconiosis, School of Public Health, China Medical University
    • , Qin-cheng HeAffiliated withDivision of Pneumoconiosis, School of Public Health, China Medical University Email author 

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Abstract

In vitro and in vivo studies have demonstrated that lung cell apoptosis is associated with lung fibrosis; however the relationship between apoptosis of alveolar macrophages (AMs) and human silicosis has not been addressed. In the present study, AM apoptosis was determined in whole-lung lavage fluid from 48 male silicosis patients, 13 male observers, and 13 male healthy volunteers. The relationships between apoptosis index (AI) and silica exposure history, soluble Fas (sFas)/membrane-bound Fas (mFas), and caspase-3/caspase-8 were analyzed. AI, mFas, and caspase-3 were significantly higher in lung lavage fluids from silicosis patients than those of observers or healthy volunteers, but the level of sFas demonstrated a decreasing trend. AI was related to silica exposure, upregulation of mFas, and activation of caspase-3 and -8, as well as influenced by smoking status after adjusting for confounding factors. These results indicate that AM apoptosis could be used as a potential biomarker for human silicosis, and the Fas/FasL pathway may regulate this process. The present data from human lung lavage samples may help to understand the mechanism of silicosis and in turn lead to strategies for preventing or treating this disease.

Keywords

Silicosis Alveolar macrophages Apoptosis Soluble Fas Membrane-bound Fas Caspase