Apoptosis

, 16:1195

Fas/FasL pathway-mediated alveolar macrophage apoptosis involved in human silicosis

Authors

  • San-qiao Yao
    • Division of Pneumoconiosis, School of Public HealthChina Medical University
    • Department of Occupational and Environmental Health, School of Public HealthHebei United University
    • Key Lab for Coal Mine Health and Safety of Hebei Province
  • Liying Wang Rojanasakul
    • Pathology and Physiology Research BranchNational Institute for Occupational Safety and Health (NIOSH)
  • Zhi-yuan Chen
    • Department of PneumoconiosisBeidaihe Sanitarium for China Coal Miners
  • Ying-jun Xu
    • Department of Occupational and Environmental Health, School of Public HealthHebei United University
    • Key Lab for Coal Mine Health and Safety of Hebei Province
  • Yu-ping Bai
    • Department of Occupational and Environmental Health, School of Public HealthHebei United University
    • Key Lab for Coal Mine Health and Safety of Hebei Province
  • Gang Chen
    • Department of PneumoconiosisBeidaihe Sanitarium for China Coal Miners
  • Xi-ying Zhang
    • Department of Occupational and Environmental Health, School of Public HealthHebei United University
  • Chun-min Zhang
    • Department of Occupational and Environmental Health, School of Public HealthHebei United University
    • Key Lab for Coal Mine Health and Safety of Hebei Province
  • Yan-qin Yu
    • Department of Occupational and Environmental Health, School of Public HealthHebei United University
  • Fu-hai Shen
    • Department of Occupational and Environmental Health, School of Public HealthHebei United University
    • Key Lab for Coal Mine Health and Safety of Hebei Province
  • Ju-xiang Yuan
    • Department of Occupational and Environmental Health, School of Public HealthHebei United University
    • Key Lab for Coal Mine Health and Safety of Hebei Province
  • Jie Chen
    • Division of Pneumoconiosis, School of Public HealthChina Medical University
    • Division of Pneumoconiosis, School of Public HealthChina Medical University
Original Paper

DOI: 10.1007/s10495-011-0647-4

Cite this article as:
Yao, S., Rojanasakul, L.W., Chen, Z. et al. Apoptosis (2011) 16: 1195. doi:10.1007/s10495-011-0647-4

Abstract

In vitro and in vivo studies have demonstrated that lung cell apoptosis is associated with lung fibrosis; however the relationship between apoptosis of alveolar macrophages (AMs) and human silicosis has not been addressed. In the present study, AM apoptosis was determined in whole-lung lavage fluid from 48 male silicosis patients, 13 male observers, and 13 male healthy volunteers. The relationships between apoptosis index (AI) and silica exposure history, soluble Fas (sFas)/membrane-bound Fas (mFas), and caspase-3/caspase-8 were analyzed. AI, mFas, and caspase-3 were significantly higher in lung lavage fluids from silicosis patients than those of observers or healthy volunteers, but the level of sFas demonstrated a decreasing trend. AI was related to silica exposure, upregulation of mFas, and activation of caspase-3 and -8, as well as influenced by smoking status after adjusting for confounding factors. These results indicate that AM apoptosis could be used as a potential biomarker for human silicosis, and the Fas/FasL pathway may regulate this process. The present data from human lung lavage samples may help to understand the mechanism of silicosis and in turn lead to strategies for preventing or treating this disease.

Keywords

Silicosis Alveolar macrophages Apoptosis Soluble Fas Membrane-bound Fas Caspase

Copyright information

© Springer Science+Business Media, LLC 2011