Apoptosis

, Volume 15, Issue 2, pp 204–218

Serum deprivation-induced reactive oxygen species production is mediated by Romo1

  • Seung Baek Lee
  • Jung Jin Kim
  • Tae Woo Kim
  • Byung Soo Kim
  • Myeong-Sok Lee
  • Young Do Yoo
Original Paper

DOI: 10.1007/s10495-009-0411-1

Cite this article as:
Lee, S.B., Kim, J.J., Kim, T.W. et al. Apoptosis (2010) 15: 204. doi:10.1007/s10495-009-0411-1

Abstract

Serum deprivation-triggered increases in reactive oxygen species (ROS) are known to induce apoptotic cell death. However, the mechanism by which serum deprivation causes ROS production is not known. Since mitochondria are the main source of ROS and since mitochondrial ROS modulator 1 (Romo1) is involved in ROS production, we sought to determine if serum deprivation triggered ROS production through Romo1. To examine the relationship between Romo1 and the serum deprivation-triggered increase in ROS, we transfected Romo1 siRNA into various cell lines and looked for inhibition of mitochondrial ROS generation. Romo1 knockdown by Romo1 siRNA blocked the mitochondrial ROS production caused by serum deprivation, which originates in the mitochondrial electron transport chain. We also found that Romo1 knockdown inhibited serum deprivation-induced apoptosis. These findings suggest that Romo1-derived ROS play an important role in apoptotic cell death triggered by withdrawal of cell survival factors.

Keywords

Reactive oxygen speciesRomo1Serum deprivationApoptosisMitochondria

Abbreviations

ROS

Reactive oxygen species

Romo1

Reactive oxygen species modulator 1

siRNA

Small interfering RNA

DCF-DA

2′,7′- Dichlorofluorescein diacetate

H2O2

Hydrogen peroxide

PBS

Phosphate-buffered saline

Trolox

6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid

VDAC

Voltage-dependent anion channel

FBS

Fetal bovine serum

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Seung Baek Lee
    • 1
  • Jung Jin Kim
    • 1
  • Tae Woo Kim
    • 2
  • Byung Soo Kim
    • 3
  • Myeong-Sok Lee
    • 4
  • Young Do Yoo
    • 1
  1. 1.Laboratory of Molecular Cell Biology, Graduate School of Medicine, Korea University College of MedicineKorea UniversitySeoulRepublic of Korea
  2. 2.Laboratory of Infection and Immunology, Graduate School of Medicine, Korea University College of MedicineKorea UniversitySeoulRepublic of Korea
  3. 3.Department of Internal Medicine, Korea University College of MedicineKorea UniversitySeoulRepublic of Korea
  4. 4.Department of Biological SciencesSookmyung Women’s UniversitySeoulRepublic of Korea