Apoptosis

, Volume 11, Issue 10, pp 1825–1835

Involvement of IL-10 and Bcl-2 in resistance against an asbestos-induced apoptosis of T cells

Authors

  • Yoshie Miura
    • Department of HygieneKawasaki Medical School
  • Yasumitsu Nishimura
    • Department of HygieneKawasaki Medical School
  • Hironobu Katsuyama
    • Department of Public HealthKawasaki Medical School
  • Megumi Maeda
    • Department of HygieneKawasaki Medical School
  • Hiroaki Hayashi
    • Department of HygieneKawasaki Medical School
  • Maolong Dong
    • Department of HygieneKawasaki Medical School
  • Fuminori Hyodoh
    • Department of HygieneKawasaki Medical School
  • Masafumi Tomita
    • Department of Medical ToxicologyKawasaki Medical School
  • Yoshinobu Matsuo
    • Grandsoul Research Institutes for Immunology
  • Ayuko Uesaka
    • Department of Respiratory MedicineHyogo College of Medicine
  • Kozo Kuribayashi
    • Department of Respiratory MedicineHyogo College of Medicine
  • Takashi Nakano
    • Department of Respiratory MedicineHyogo College of Medicine
  • Takumi Kishimoto
    • Okayama Rosai Hospital
    • Department of HygieneKawasaki Medical School
Report

DOI: 10.1007/s10495-006-9235-4

Cite this article as:
Miura, Y., Nishimura, Y., Katsuyama, H. et al. Apoptosis (2006) 11: 1825. doi:10.1007/s10495-006-9235-4

Abstract

To analyze the possibility that immunological alteration in asbestos-related diseases (ARDs) such as asbestosis (ASB) and malignant mesothelioma (MM) may affect the progression of cancers, a human adult T cell leukemia virus-immortalized T cell line (MT-2Org) was continuously exposed to 10 μg/ml of chrysotile-B (CB), an asbestos. After at least 8 months of exposure, the rate of apoptosis in the cells became very low and the resultant subline was designated MT-2Rst. The MT-2Rst cells were characterized by (i) enhanced expression of bcl-2, with regain of apoptosis-sensitivity by reduction of bcl-2 by siRNA, (ii) excess IL-10 secretion and expression, and (iii) activation of STAT3 that was inhibited by PP2, a specific inhibitor of Src family kinases. These results suggested that the contact between cells and asbestos may affect the human immune system and trigger a cascade of biological events such as activation of Src family kinases, enhancement of IL-10 expression, STAT3 activation and Bcl-2 overexpression. This speculation was partially confirmed by the detection of elevated bcl-2 expression levels in CD4 + peripheral blood T cells from patients with MM compared with those from patients with ASB or healthy donors. Further studies will be required to verify the role of T cells with enhanced bcl-2 expression in tumor progression induced by asbestos exposure.

Keywords

AsbestosT cellIL-10Bcl-2Apoptosis

Copyright information

© Springer Science + Business Media, LLC 2006