Apoptosis

, Volume 11, Issue 10, pp 1825–1835

Involvement of IL-10 and Bcl-2 in resistance against an asbestos-induced apoptosis of T cells

  • Yoshie Miura
  • Yasumitsu Nishimura
  • Hironobu Katsuyama
  • Megumi Maeda
  • Hiroaki Hayashi
  • Maolong Dong
  • Fuminori Hyodoh
  • Masafumi Tomita
  • Yoshinobu Matsuo
  • Ayuko Uesaka
  • Kozo Kuribayashi
  • Takashi Nakano
  • Takumi Kishimoto
  • Takemi Otsuki
Report

DOI: 10.1007/s10495-006-9235-4

Cite this article as:
Miura, Y., Nishimura, Y., Katsuyama, H. et al. Apoptosis (2006) 11: 1825. doi:10.1007/s10495-006-9235-4

Abstract

To analyze the possibility that immunological alteration in asbestos-related diseases (ARDs) such as asbestosis (ASB) and malignant mesothelioma (MM) may affect the progression of cancers, a human adult T cell leukemia virus-immortalized T cell line (MT-2Org) was continuously exposed to 10 μg/ml of chrysotile-B (CB), an asbestos. After at least 8 months of exposure, the rate of apoptosis in the cells became very low and the resultant subline was designated MT-2Rst. The MT-2Rst cells were characterized by (i) enhanced expression of bcl-2, with regain of apoptosis-sensitivity by reduction of bcl-2 by siRNA, (ii) excess IL-10 secretion and expression, and (iii) activation of STAT3 that was inhibited by PP2, a specific inhibitor of Src family kinases. These results suggested that the contact between cells and asbestos may affect the human immune system and trigger a cascade of biological events such as activation of Src family kinases, enhancement of IL-10 expression, STAT3 activation and Bcl-2 overexpression. This speculation was partially confirmed by the detection of elevated bcl-2 expression levels in CD4 + peripheral blood T cells from patients with MM compared with those from patients with ASB or healthy donors. Further studies will be required to verify the role of T cells with enhanced bcl-2 expression in tumor progression induced by asbestos exposure.

Keywords

AsbestosT cellIL-10Bcl-2Apoptosis

Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Yoshie Miura
    • 1
  • Yasumitsu Nishimura
    • 1
  • Hironobu Katsuyama
    • 2
  • Megumi Maeda
    • 1
  • Hiroaki Hayashi
    • 1
  • Maolong Dong
    • 1
  • Fuminori Hyodoh
    • 1
  • Masafumi Tomita
    • 3
  • Yoshinobu Matsuo
    • 4
  • Ayuko Uesaka
    • 5
  • Kozo Kuribayashi
    • 5
  • Takashi Nakano
    • 5
  • Takumi Kishimoto
    • 6
  • Takemi Otsuki
    • 1
  1. 1.Department of HygieneKawasaki Medical SchoolOkayamaJapan
  2. 2.Department of Public HealthKawasaki Medical SchoolOkayamaJapan
  3. 3.Department of Medical ToxicologyKawasaki Medical SchoolOkayamaJapan
  4. 4.Grandsoul Research Institutes for ImmunologyNaraJapan
  5. 5.Department of Respiratory MedicineHyogo College of MedicineHyogoJapan
  6. 6.Okayama Rosai HospitalOkayamaJapan