Apoptosis

, Volume 11, Issue 4, pp 535–544

Ultrastructural characterization of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-induced cell death in embryonic dopaminergic neurons

Authors

  • D. A. Dorsey
    • Department of PsychiatryWashington University School of Medicine
  • D. H. Mascó
    • Department of PsychiatryWashington University School of Medicine
    • Facultad de Ciencias QuímicasUniversidad Nacional de Córdoba
  • K. Dikranian
    • Department of PsychiatryWashington University School of Medicine
  • K. Hyrc
    • Department of NeurologyWashington University School of Medicine
  • L. Masciotra
    • Department of PsychiatryWashington University School of Medicine
  • B. Faddis
    • Department of OtolaryngologyWashington University School of Medicine
  • M. Soriano
    • Departamento de Biología CelularUniversidad de Valencia
  • A. A. Gru
    • Department of PsychiatryWashington University School of Medicine
  • M. P. Goldberg
    • Departamento de Biología CelularUniversidad de Valencia
    • Hope Center for Neurological DisordersWashington University School of Medicine
    • Department of PsychiatryWashington University School of Medicine
    • Department of NeurologyWashington University School of Medicine
    • Hope Center for Neurological DisordersWashington University School of Medicine
    • Psychiatry and NeurologyWashington University School of Medicine
Reports

DOI: 10.1007/s10495-006-5268-y

Cite this article as:
Dorsey, D.A., Mascó, D.H., Dikranian, K. et al. Apoptosis (2006) 11: 535. doi:10.1007/s10495-006-5268-y

Abstract

Developing neuronal populations undergo significant attrition by natural cell death. Dopaminergic neurons in the substantia nigra pars compacta undergo apoptosis during synaptogenesis. Following this time window, destruction of the anatomic target of dopaminergic neurons results in dopaminergic cell death but the morphology is no longer apoptotic. We describe ultrastructural changes that appear unique to dying embryonic dopaminergic neurons. In primary cultures of mesencephalon, death of dopaminergic neurons is triggered by activation of glutamate receptors sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), and differs ultrastructurally from both neuronal apoptosis or typical excitotoxicity. AMPA causes morphological changes selectively in dopaminergic neurons, without affecting other neurons in the same culture dishes. Two hours after the onset of treatment swelling of Golgi complexes is apparent. At 3 h, dopaminergic neurons display loss of membrane asymmetry (coinciding with commitment to die), as well as nuclear membrane invagination, irregular aggregation of chromatin, and mitochondrial swelling. Nuclear changes continue to worsen until loss of cytoplasmic structures and cell death begins to occur after 12 h. These changes are different from those described in neurons undergoing either apoptosis or excitotoxic death, but are similar to ultrastructural changes observed in spontaneous death of dopaminergic neurons in the natural mutant weaver mouse.

Keywords

alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid programmed cell death glutamate receptors ultrastructure

Copyright information

© Springer Science + Business Media, LLC 2006