Apoptosis

, Volume 12, Issue 6, pp 1061–1068

A label-free photonic crystal biosensor imaging method for detection of cancer cell cytotoxicity and proliferation

  • Leo L. Chan
  • Saujanya L. Gosangari
  • Kenneth L. Watkin
  • Brian T. Cunningham
Article

DOI: 10.1007/s10495-006-0031-y

Cite this article as:
Chan, L.L., Gosangari, S.L., Watkin, K.L. et al. Apoptosis (2007) 12: 1061. doi:10.1007/s10495-006-0031-y

Abstract

A label-free method for detecting the attachment of human cancer cells to a biosensor surface for rapid screening for biological activity is described, in which attachment of a cell results in highly localized increase of the resonant reflected wavelength of a photonic crystal narrowband reflectance filter incorporated into a standard 96-well microplate. An imaging detection instrument is used to determine the spatial distribution of attached cells by mapping the shift in reflected resonant wavelength as a function of position. The method enables monitoring of cancer cell attachment, cell proliferation, and cell detachment that is induced by exposure of the cells to drug compounds. We demonstrate the efficacy of this method as an early screening technique for the rapid quantification of the rate of cancer cell proliferation on the sensor surface, and subsequently as a means for quantifying cell detachment resulting from apoptosis that is induced by exposure of the cells to cytotoxic chemicals.

Keywords

Biosensor Label-free detection Cell imaging 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Leo L. Chan
    • 1
  • Saujanya L. Gosangari
    • 2
    • 3
  • Kenneth L. Watkin
    • 2
    • 3
  • Brian T. Cunningham
    • 1
    • 4
  1. 1.Department of Electrical and Computer EngineeringUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.Beckman Institute for Advanced Science and TechnologyBio-Imaging Science and Technology Group, University of Illinois at Urbana-ChampaignUrbanaUSA
  3. 3.Department of Speech and Hearing ScienceUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  4. 4.Micro and Nanotechnology LaboratoryUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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