Experimental and Applied Acarology

, Volume 60, Issue 1, pp 63–72

Ixodid fauna and zoonotic agents in ticks from dogs: first report of Rickettsia rickettsii in Rhipicephalus sanguineus in the state of Mato Grosso do Sul, mid-western Brazil

  • Robson Ferreira Cavalcante de Almeida
  • Marcos Valério Garcia
  • Rodrigo Casquero Cunha
  • Jaqueline Matias
  • Elaine Araújo e Silva
  • Maria de Fatima Cepa Matos
  • Renato Andreotti
Article

DOI: 10.1007/s10493-012-9641-y

Cite this article as:
de Almeida, R.F.C., Garcia, M.V., Cunha, R.C. et al. Exp Appl Acarol (2013) 60: 63. doi:10.1007/s10493-012-9641-y

Abstract

Ticks from 148 dogs from the urban area of the municipality of Campo Grande, state of Mato Grosso do Sul, Brazil, were collected, classified and analyzed using polymerase chain reaction (PCR) for the identification of Rickettsia spp., Trypanosoma cruzi and Leishmania spp. A total of 2015 ticks were collected. The species Rhipicephalus sanguineus (98.9 %) and Amblyomma cajennense (1.1 %) were identified. Molecular analysis revealed that no tick samples were infected by T. cruzi. Regarding Leishmania spp., tick samples from 36 dogs spread across all regions of the municipality were positive for L. chagasi. One tick sample was positive for Rickettsia spp. (gltA gene) in the PCR reaction. This sample was submitted to further PCR based on the ompA gene and the amplicon was sequenced. Identity of 100 % was found with homologous sequences of R. rickettsii available in GenBank. This paper is the first to report the natural infection of R. sanguineus by R. rickettsii in the municipality of Campo Grande, state of Mato Grosso do Sul, mid-western Brazil.

Keywords

Survey of ticks Zoonosis Spotted fever Molecular detection 

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Robson Ferreira Cavalcante de Almeida
    • 1
  • Marcos Valério Garcia
    • 2
  • Rodrigo Casquero Cunha
    • 3
  • Jaqueline Matias
    • 3
  • Elaine Araújo e Silva
    • 4
  • Maria de Fatima Cepa Matos
    • 1
  • Renato Andreotti
    • 2
  1. 1.Programa de Pós-graduação em Doenças Infecciosas e Parasitárias, Faculdade de MedicinaUniversidade Federal de Mato Grosso do SulCampo GrandeBrazil
  2. 2.Embrapa Gado de CorteCampo GrandeBrazil
  3. 3.Programa de Pós-graduação em Ciência Animal, Faculdade de Medicina Veterinária e ZootecniaUniversidade Federal de Mato Grosso do SulCampo GrandeBrazil
  4. 4.Centro de Controle de ZoonosesPrefeitura Municipal deCampo GrandeBrazil

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