Experimental and Applied Acarology

, 50:163

Predominance of Ehrlichia chaffeensis in Rhipicephalus sanguineus ticks from kennel-confined dogs in Limbe, Cameroon

Authors

    • Department of Biochemistry and MicrobiologyUniversity of Buea
  • Roland N. Ndip
    • Department of Biochemistry and MicrobiologyUniversity of Fort Hare
  • Seraphine N. Esemu
    • Department of Biochemistry and MicrobiologyUniversity of Buea
  • David H. Walker
    • Department of PathologyUniversity of Texas Medical Branch
    • Center for Biodefense and Emerging Infectious DiseasesUniversity of Texas Medical Branch
  • Jere W. McBride
    • Department of PathologyUniversity of Texas Medical Branch
    • Center for Biodefense and Emerging Infectious DiseasesUniversity of Texas Medical Branch
    • Department of Microbiology and ImmunologyUniversity of Texas Medical Branch
Article

DOI: 10.1007/s10493-009-9293-8

Cite this article as:
Ndip, L.M., Ndip, R.N., Esemu, S.N. et al. Exp Appl Acarol (2010) 50: 163. doi:10.1007/s10493-009-9293-8

Abstract

Rhipicephalus sanguineus ticks (n = 63) collected from five dogs (two adults and three puppies) housed in a kennel were screened for Ehrlichial agents (Ehrlichia canis, E. chaffeensis, and E. ewingii) using a species-specific multicolor real-time TaqMan PCR amplification of the disulphide bond formation protein (dsb) gene. Ehrlichia chaffeensis DNA was detected in 33 (56%) ticks, E. canis DNA was detected in four (6%) ticks, and one tick was coinfected. The E. chaffeensis and E. canis nucleotide sequences of the amplified dsb gene (374 bp) obtained from the Cameroonian R. sanguineus ticks were identical to the North American genotypes.

Keywords

Rhipicephalus sanguineusEhrlichia chaffeensisEhrlichia canisZoonosisCameroon

Copyright information

© Springer Science+Business Media B.V. 2009