Abstract
A bacterial strain, designated LYBFD3-16A2T, was isolated from tribenuron methyl contaminated wheat soil. Cells were observed to be Gram-negative short rods with a single flagellum. The strain was found to utilize methanol, glucose, maltose and mannitol as carbon and energy sources, and utilized glutamate, leucine, phenylalanine as organic nitrogen sources. Strain LYBFD3-16A2T was found to be aerobic, to form urease, produce hydrogen sulfide and reduce nitrate to nitrite. The indole test in tryptone broth was observed to be positive. The major cellular fatty acids were identified as C18:1ω7c (81.3 %), 11-methylC18:1ω7c (7.9 %), C18:0 (3.0 %) and C16:0 (3.0 %). The major phospholipids were identified as phosphatidylcholine, phosphatidylethanolamine, phosphatidyglycerol and diphosphatidylglycerol. The main ubiquinone was identified as Q-10. The DNA G+C content was determined to be between 70.2 and 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated the affiliation of strain LYBFD3-16A2 to members of the genus Methylopila. The DNA–DNA hybridization values of the novel strain with the type strains of the most closely related species Methylopila musalis MUSAT and Methylopila jiangsuensis JZL-4T were 35.4 % and 31.4 % respectively. The genotypic and phenotypic characterization, along with chemotaxonomic properties of strain LYBFD3-16A2T, showed that the strain represents a novel species of the genus Methylopila for which the name Methylopila henanense sp. nov. is proposed. The type strain is LYBFD3-16A2T (=CGMCC1.10703T = LMG 25959T).
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Acknowledgments
The authors are indebted to Professor Yan Xiao-Jun (Ningbo University, China) and Professor Henglin Cui (School of Food & Biological Engineering, Jiangsu University) for their valuable help. This work was supported by the National Natural Science Foundation of China (Grant: 31100582) and by the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant KSCX2-YW-G-052).
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain LYBFD3-16A2T is HM447243.
Ya-Nan Wang and Wen-Yu Tian have been contributed equally to this work.
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Wang, YN., Tian, WY., He, WH. et al. Methylopila henanense sp. nov., a novel methylotrophic bacterium isolated from tribenuron methyl-contaminated wheat soil. Antonie van Leeuwenhoek 107, 329–336 (2015). https://doi.org/10.1007/s10482-014-0331-0
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DOI: https://doi.org/10.1007/s10482-014-0331-0