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Gracilibacillus xinjiangensis sp. nov., a new member of the genus Gracilibacillus isolated from Xinjiang region, China

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Abstract

A Gram-positive, endospore-forming, rod-shaped bacterium, designated isolate J2T was isolated from a soil sample from Xinjiang Uyghur Autonomous Region, China. The isolate was observed to grow at 16–46 °C and pH 6.5–8.0. Chemotaxonomic analysis showed menaquinone-7 (MK-7) to be the major isoprenoid quinone; diphosphatidylglycerol, phosphatidylglycerol, one aminophospholipid, two phosphoglycolipids and one glycolipid as the major cellular polar lipids; and anteiso-C15:0, iso-C15:0, anteiso-C17:0 and C16:0 as the major fatty acids. Comparative analyses of the 16S rRNA gene sequence showed that strain J2T is most closely related to Gracilibacillus ureilyticus (with 98.8 % similarity), Gracilibacillus dipsosauri (97.2 %), Gracilibacillus quinghaiensis (97.1 %) and Gracilibacillus thailandensis (97.0 %). The DNA–DNA reassociation values between strain J2T and G. ureilyticus MF38T, G. dipsosauri DD1T, G. quinghaiensis YIM-C229T and G. thailandensis TP2-8T were 29.8 ± 3.7, 23.0 ± 3.5, 15.8 ± 4.9 and 15.9 ± 5.0 %, respectively. The genomic DNA G+C content of strain J2T was determined to be 36.5 mol%. Based on these data, strain J2T is considered as a novel species of the genus Gracilibacillus, for which the name Gracilibacillus xinjiangensis sp. nov. is proposed. The type species is J2T (= CGMCC 1.12449T = JCM 18859T).

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Acknowledgments

The authors are very grateful for the technical support provided by Yuqin Zhang, Jingnan Liang, Hongcan Liu. This work was supported in part by grants from the Ministry of Science and Technology of China (2013CB734000, 2012CB725200, 2012CB721006, 2011ZX11102-011-11), National Natural Science Foundation of China (31100075, 812111049, 31170095, 31000004) and the CAS Pillar Program (XDA04074000) and. L. Z. is an Awardee for National Distinguished Young Scholar Program in China.

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Authors and Affiliations

  1. Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100190, People’s Republic of China

    Na Yang, Biao Ren, Huanqin Dai, Zhiheng Liu, Fuhang Song & Lixin Zhang

  2. Graduate School of Chinese Academy of Sciences (or University of Chinese Academy of Sciences), Beijing, 100049, People’s Republic of China

    Na Yang & Biao Ren

  3. China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Yuguang Zhou

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  1. Na Yang
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  2. Biao Ren
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  3. Huanqin Dai
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  4. Zhiheng Liu
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  5. Yuguang Zhou
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  6. Fuhang Song
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Corresponding authors

Correspondence to Fuhang Song or Lixin Zhang.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain J2T is JQ911669.

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Yang, N., Ren, B., Dai, H. et al. Gracilibacillus xinjiangensis sp. nov., a new member of the genus Gracilibacillus isolated from Xinjiang region, China. Antonie van Leeuwenhoek 104, 809–816 (2013). https://doi.org/10.1007/s10482-013-9992-3

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