Antonie van Leeuwenhoek

, Volume 103, Issue 6, pp 1395–1404

Identification of up-regulated proteins potentially involved in the antagonism mechanism of Bacillus amyloliquefaciens G1

Short Communication

DOI: 10.1007/s10482-013-9902-8

Cite this article as:
Cao, H., Zheng, W., He, S. et al. Antonie van Leeuwenhoek (2013) 103: 1395. doi:10.1007/s10482-013-9902-8


The use of Bacillus probiotics has been demonstrated as a promising method in the biocontrol of bacterial diseases in aquaculture. However, the molecular antibacterial mechanism of Bacillus still remains unclear. In order to explore the antibacterial mechanism of the potential antagonistic Bacillus amyloliquefaciens strain G1, comparative proteomics between B. amyloliquefaciens strain G1 and its non-antagonistic mutant strain was investigated. The 2-dimensional electrophoresis gel maps of their total extracted proteins were described and 42 different proteins were found to be highly expressed in strain G1 in comparison with those in the mutant strain. 35 of these up-regulated proteins were successfully identified using MALDI-TOF-TOF MS and databank analysis, and their biological functions were analyzed through the KEGG database. The increased expression of these proteins suggested that high levels of energy metabolism, biosynthesis and stress resistance could play important roles in strain G1’s antagonism. To our knowledge, this is the first report on the proteins involved in the antagonism mechanism of B. amyloliquefaciens using a proteomic approach and the proteomic data also contribute to a better understanding of the molecular basis for the antagonism of B. amyloliquefaciens.


Bacillus amyloliquefaciensAntagonism mechanismProteomicsUp-regulated proteins

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources of Ministry of EducationShanghai Ocean UniversityShanghaiPeople’s Republic of China
  2. 2.National Pathogen Collection Center for Aquatic AnimalsShanghai Ocean UniversityShanghaiPeople’s Republic of China
  3. 3.Yangtze River Fisheries Research Institute, Chinese Academy of Fishery SciencesWuhanPeople’s Republic of China