Antonie van Leeuwenhoek

, Volume 103, Issue 3, pp 647–653

Paenibacillus brassicae sp. nov., isolated from cabbage rhizosphere in Beijing, China

Authors

  • Miao Gao
    • Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences
  • Hui Yang
    • Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western ChinaSchool of Life Science, Ningxia University
  • Ji Zhao
    • School of Life Science, Neimenggu University
  • Jun Liu
    • School of Life Science, Neimenggu University
  • Yan-hua Sun
    • School of Life Science, Neimenggu University
  • Yu-jiong Wang
    • Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western ChinaSchool of Life Science, Ningxia University
    • Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences
Original Paper

DOI: 10.1007/s10482-012-9849-1

Cite this article as:
Gao, M., Yang, H., Zhao, J. et al. Antonie van Leeuwenhoek (2013) 103: 647. doi:10.1007/s10482-012-9849-1

Abstract

A novel Gram-positive, rod-shaped, motile, spore-forming, nitrogen-fixing bacterium, designated strain 112T, was isolated from cabbage rhizosphere in Beijing, China. The strain was found to grow at 10–40 °C and pH 4–11, with an optimum of 30 °C and pH 7.0, respectively. Phylogenetic analysis based on a fragment of the full-length 16S rRNA gene sequence revealed that strain 112T is a member of the genus Paenibacillus. High levels of 16S rRNA gene similarities were found between strain 112T, Paenibacillus sabinae DSM 17841T (97.82 %) and Paenibacillus forsythiae DSM 17842T (97.22 %). However, the DNA–DNA hybridization values between strain 112T and the type strains of these two species were 10.36 and 6.28 %, respectively. The predominant menaquinone was found to be menaquinone 7 (MK-7). The major fatty acids were determined to be anteiso-C15:0 and C16:0. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unknown aminophospholipids. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. The DNA G+C content was determined to be 55.4 mol%. On the basis of its phenotypic characteristics, 16S rRNA gene sequence analysis and the value of DNA–DNA hybridization, strain 112T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus brassicae sp. nov. is proposed. The type strain is 112T (= ACCC 01125T = DSM 24983T).

Keywords

Paenibacillus brassicae sp. nov.Nitrogen-fixationCabbage rhizosphere

Supplementary material

10482_2012_9849_MOESM1_ESM.docx (80 kb)
Supplementary material 1 (DOCX 80 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2012