Antonie van Leeuwenhoek

, Volume 103, Issue 3, pp 513–518

Thermus tengchongensis sp. nov., isolated from a geothermally heated soil sample in Tengchong, Yunnan, south-west China

  • Tian-Tian Yu
  • Ji-Cheng Yao
  • Hong Ming
  • Yi-Rui Yin
  • En-Min Zhou
  • Min-Jiao Liu
  • Shu-Kun Tang
  • Wen-Jun Li
Original Paper

DOI: 10.1007/s10482-012-9833-9

Cite this article as:
Yu, TT., Yao, JC., Ming, H. et al. Antonie van Leeuwenhoek (2013) 103: 513. doi:10.1007/s10482-012-9833-9

Abstract

A Gram-stain negative aerobic bacterium, designated YIM 77924T, was isolated from a geothermally heated soil sample collected at Rehai National Park, Tengchong, Yunnan province, south-west China. Growth was found to occur from 55 to 75 °C (optimum 65 °C), pH 6.0–8.0 (optimum pH 7.0) and 0–1 % NaCl (w/v). Cells were observed to be rod-shaped and the colonies convex, circular, smooth, yellow and non-transparent. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM 77924T belongs to the genus Thermus. The 16S rRNA gene sequence similarity values between strain YIM 77924T and other species of the genus Thermus were all below 97 %. The polar lipids of strain YIM 77924T were determined to be aminophospholipid, phospholipid and glycolipid. The predominant respiratory quinone was determined to be MK-8 and the G+C content was 66.64 mol%. The major fatty acids identified were iso-C16:0, iso-C15:0, iso-C17:0 and C16:0. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain YIM 77924T is proposed to represent a novel species, Thermus tengchongensis sp. nov., in the genus Thermus. The type strain is YIM 77924T (=KCTC 32025T = CCTCC AB2012063T).

Keywords

Thermophile Thermus tengchongensis sp. nov. 16S rRNA gene Polyphasic taxonomy 

Supplementary material

10482_2012_9833_MOESM1_ESM.doc (8.2 mb)
Supplementary material 1 (DOC 8348 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Tian-Tian Yu
    • 1
    • 2
  • Ji-Cheng Yao
    • 2
  • Hong Ming
    • 2
    • 3
  • Yi-Rui Yin
    • 2
  • En-Min Zhou
    • 2
  • Min-Jiao Liu
    • 2
  • Shu-Kun Tang
    • 2
  • Wen-Jun Li
    • 1
    • 2
  1. 1.Key Laboratory of Biogeography and Bioresource in Arid Land, CASXinjiang Institute of Ecology and Geography, Chinese Academy of SciencesÜrűmqiPeople’s Republic of China
  2. 2.Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resourcesYunnan Institute of Microbiology, Yunnan UniversityKunmingPeople’s Republic of China
  3. 3.Department of Life Sciences and TechnologyXinxiang Medical UniversityXinxiangPeople’s Republic of China

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