Antonie van Leeuwenhoek

, Volume 103, Issue 3, pp 457–464

Lentilitoribacterdonghaensis gen. nov., sp. nov., a slowly-growing alphaproteobacterium isolated from coastal seawater

  • Sooyeon Park
  • Jung-Sook Lee
  • Kenu-Chul Lee
  • Jung-Hoon Yoon
Original Paper

DOI: 10.1007/s10482-012-9825-9

Cite this article as:
Park, S., Lee, JS., Lee, KC. et al. Antonie van Leeuwenhoek (2013) 103: 457. doi:10.1007/s10482-012-9825-9

Abstract

A Gram-staining-negative, aerobic, non-spore-forming, non-flagellated and rod- or short rod-shaped bacterial strain, designated BH-4T, was isolated from coastal seawater in the East Sea, South Korea. Strain BH-4T grew optimally at 25 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. Strain BH-4T exhibited the highest 16S rRNA gene sequence similarity value (96.5 %) to Hoefleaphototrophica NCIMB 14078T. It exhibited 94.2–95.5, 94.7 and 93.8 % sequence similarity values to the type strains of the other Hoeflea species, Ahrensia kielensis and Pseudahrensia aquimaris, respectively. Neighbor-joining, maximum-likelihood and maximum-parsimony phylogenetic trees revealed that strain BH-4T clustered with the type strains of A. kielensis and P. aquimaris. Strain BH-4T contained Q-10 as the predominant ubiquinone as shown in the genera Ahrensia, Pseudahrensia and Hoeflea. The fatty acid and polar lipid profiles of strain BH-4T were distinguished from those of members of the genera Ahrensia, Pseudahrensia and Hoeflea. The DNA G + C content was 49.3 mol%, lower than those of the type strains of P. aquimaris and Hoeflea species. The phylogenetic data, differential chemotaxonomic and other phenotypic properties revealed that strain BH-4T constitutes a new genus and species within the Alphaproteobacteria, for which the name Lentilitoribacterdonghaensis gen. nov., sp. nov., is proposed. The type strain of Lentilitoribacterdonghaensis is BH-4T (=KCTC 32082T = CCUG 62792T).

Keywords

LentilitoribacterdonghaensisNovel genus and speciesPolyphasic taxonomyCoastal seawater

Supplementary material

10482_2012_9825_MOESM1_ESM.ppt (127 kb)
Supplementary Fig. 1Maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showing the positions of Lentilitoribacter donghaensis BH-4T and representatives of some other related taxa. Bootstrap values (expressed as percentages of 1000 replications) of >50 % are shown at branching points. Hyphomicrobium denitrificans DSM1869T was used as an outgroup. Bar, 0.01 substitutions per nucleotide position (PPT 127 kb)
10482_2012_9825_MOESM2_ESM.ppt (128 kb)
Supplementary Fig. 2Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences showing the positions of Lentilitoribacter donghaensis BH-4T and representatives of some other related taxa. Bootstrap values (expressed as percentages of 1000 replications) of >50 % are shown at branching points. Hyphomicrobium denitrificans DSM1869T was used as an outgroup. Bar, 0.01 substitutions per nucleotide position (PPT 128 kb)
10482_2012_9825_MOESM3_ESM.ppt (146 kb)
Supplementary Fig. 3Thin layer chromatogram of the total polar lipids of Lentilitoribacter donghaensis BH-4T. Spots were revealed by spraying the plates with 10 % ethanolic molybdophosphoric acid. Abbreviations: PG, phosphatidylglycerol; PME, phosphatidylmonomethylethanolamine; AL, unidentified aminolipid; PL, unidentified phospholipid (PPT 146 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Sooyeon Park
    • 1
  • Jung-Sook Lee
    • 2
  • Kenu-Chul Lee
    • 2
  • Jung-Hoon Yoon
    • 1
  1. 1.Department of Food Science and BiotechnologySungkyunkwan UniversitySuwonSouth Korea
  2. 2.Biological Resource CenterKorea Research Institute of Bioscience and Biotechnology (KRIBB)Yuseong, DaejeonSouth Korea