Original Paper

Antonie van Leeuwenhoek

, Volume 103, Issue 2, pp 399-408

Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae

  • Feiyu JiaAffiliated withSchool of Life Science, Northeast Agricultural University
  • , Chongxi LiuAffiliated withSchool of Life Science, Northeast Agricultural University
  • , Xiangjing WangAffiliated withSchool of Life Science, Northeast Agricultural University
  • , Junwei ZhaoAffiliated withSchool of Life Science, Northeast Agricultural University
  • , Qianfeng LiuAffiliated withSchool of Life Science, Northeast Agricultural University
  • , Ji ZhangAffiliated withSchool of Life Science, Northeast Agricultural University
  • , Ruixia GaoAffiliated withSchool of Life Science, Northeast Agricultural University
  • , Wensheng XiangAffiliated withSchool of Life Science, Northeast Agricultural University Email author 

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Abstract

A novel endophytic actinomycete, designated strain NEAU-J3T, was isolated from soybean root (Glycine max (L.) Merr) and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain NEAU-J3T fell within the family Micromonosporaceae. The strain was observed to form an extensively branched substrate mycelium, which carried non-motile oval spores with a smooth surface. The cell walls of strain NEAU-J3T were determined to contain meso-diaminopimelic acid and galactose, ribose and glucose were detected as whole-cell sugars. The major menaquinones were determined to be MK-9(H4) and MK-9(H6). The phospholipids detected were phosphatidylcholine and phosphatidylethanolamine. The major cellular fatty acids were determined to be C16:0, C18:1 ω9c, C18:0, C17:0, C17:1 ω7c, anteiso-C17:0, C16:1 ω7c and C15:0. The DNA G + C content was 62.5 mol%. On the basis of the morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain NEAU-J3T is considered to represent a novel species of a new genus within the family Micromonosporaceae, for which the name Wangella harbinensis gen. nov., sp. nov. is proposed. The type strain of Wangella harbinensis is strain NEAU-J3T (=CGMCC 4.7039T = DSM 45747T).

Keywords

Wangella harbinensis gen. nov. Sp. nov. Polyphasic taxonomy 16S rRNA gene