Antonie van Leeuwenhoek

, Volume 103, Issue 2, pp 399–408

Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae

  • Feiyu Jia
  • Chongxi Liu
  • Xiangjing Wang
  • Junwei Zhao
  • Qianfeng Liu
  • Ji Zhang
  • Ruixia Gao
  • Wensheng Xiang
Original Paper

DOI: 10.1007/s10482-012-9820-1

Cite this article as:
Jia, F., Liu, C., Wang, X. et al. Antonie van Leeuwenhoek (2013) 103: 399. doi:10.1007/s10482-012-9820-1

Abstract

A novel endophytic actinomycete, designated strain NEAU-J3T, was isolated from soybean root (Glycine max (L.) Merr) and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain NEAU-J3T fell within the family Micromonosporaceae. The strain was observed to form an extensively branched substrate mycelium, which carried non-motile oval spores with a smooth surface. The cell walls of strain NEAU-J3T were determined to contain meso-diaminopimelic acid and galactose, ribose and glucose were detected as whole-cell sugars. The major menaquinones were determined to be MK-9(H4) and MK-9(H6). The phospholipids detected were phosphatidylcholine and phosphatidylethanolamine. The major cellular fatty acids were determined to be C16:0, C18:1 ω9c, C18:0, C17:0, C17:1 ω7c, anteiso-C17:0, C16:1 ω7c and C15:0. The DNA G + C content was 62.5 mol%. On the basis of the morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain NEAU-J3T is considered to represent a novel species of a new genus within the family Micromonosporaceae, for which the name Wangella harbinensis gen. nov., sp. nov. is proposed. The type strain of Wangella harbinensis is strain NEAU-J3T (=CGMCC 4.7039T = DSM 45747T).

Keywords

Wangella harbinensis gen. nov. Sp. nov.Polyphasic taxonomy16S rRNA gene

Supplementary material

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Feiyu Jia
    • 1
  • Chongxi Liu
    • 1
  • Xiangjing Wang
    • 1
  • Junwei Zhao
    • 1
  • Qianfeng Liu
    • 1
  • Ji Zhang
    • 1
  • Ruixia Gao
    • 1
  • Wensheng Xiang
    • 1
  1. 1.School of Life ScienceNortheast Agricultural UniversityHarbinPeople’s Republic of China