Antonie van Leeuwenhoek

, Volume 101, Issue 3, pp 479–492

A novel function of Streptomyces integration host factor (sIHF) in the control of antibiotic production and sporulation in Streptomyces coelicolor

Authors

  • Yung-Hun Yang
    • Department of Microbial EngineeringCollege of Engineering, Konkuk University
  • Eunjung Song
    • School of Chemical and Biological Engineering and Institute of BioengineeringSeoul National University
  • Joost Willemse
    • Molecular Biotechnology, Leiden Institute of ChemistryLeiden University
  • Sung-Hee Park
    • School of Chemical and Biological Engineering and Institute of BioengineeringSeoul National University
  • Woo-Seong Kim
    • School of Chemical and Biological Engineering and Institute of BioengineeringSeoul National University
  • Eun-jung Kim
    • School of Chemical and Biological Engineering and Institute of BioengineeringSeoul National University
  • Bo-Rahm Lee
    • School of Chemical and Biological Engineering and Institute of BioengineeringSeoul National University
  • Ji-Nu Kim
    • School of Chemical and Biological Engineering and Institute of BioengineeringSeoul National University
  • Gilles P. van Wezel
    • Molecular Biotechnology, Leiden Institute of ChemistryLeiden University
    • School of Chemical and Biological Engineering and Institute of BioengineeringSeoul National University
Original Paper

DOI: 10.1007/s10482-011-9657-z

Cite this article as:
Yang, Y., Song, E., Willemse, J. et al. Antonie van Leeuwenhoek (2012) 101: 479. doi:10.1007/s10482-011-9657-z

Abstract

Bacterial integration host factors (IHFs) play important roles in site-specific recombination, DNA replication, transcription, genome organization and bacterial pathogenesis. In Streptomyces coelicolor, there are three putative IHFs: SCO1480, SCO2950 and SCO5556. SCO1480 or Streptomyces IHF (sIHF) was previously identified as a transcription factor that binds to the promoter region of redD, the pathway-specific regulatory gene for the undecylprodigiosin biosynthetic gene cluster. Here we show that production of the pigmented antibiotics actinorhodin and undecylprodigiosin is strongly enhanced in sihf null mutants, while sporulation was strongly inhibited, with an on average 25% increase in spore size. Furthermore, the sihf mutant spores showed strongly reduced viability, with high sensitivity to heat and live/dead staining revealing a high proportion of empty spores, while enhanced expression of sIHF increased viability. This suggests a major role for sIHF in controlling viability, perhaps via the control of DNA replication and/or segregation. Proteomic analysis of the sihf null mutant identified several differentially expressed transcriptional regulators, indicating that sIHF may have an extensive response regulon. These data surprisingly reveal that a basic architectural element conserved in many actinobacteria such as mycobacteria, corynebacteria, streptomycetes and rhodococci may act as a global regulator of secondary metabolism and cell development.

Keywords

DNA replication Chromosome segregation Development Nucleoid HU IHF

Supplementary material

10482_2011_9657_MOESM1_ESM.doc (66 kb)
Supplementary material 1 (DOC 66 kb)

Copyright information

© Springer Science+Business Media B.V. 2011