Antonie van Leeuwenhoek

, Volume 101, Issue 1, pp 133–140

Tunicatimonas pelagia gen. nov., sp. nov., a novel representative of the family Flammeovirgaceae isolated from a sea anemone by the differential growth screening method

Authors

    • College of PharmacyKeimyung University
  • Naoya Oku
    • Marine Biotechnology Institute Co. Ltd
    • Biotechnology Research CenterToyama Prefectural University
  • Sanghwa Park
    • Institute of Molecular and Cellular BiosciencesThe University of Tokyo
  • Atsuko Katsuta
    • Marine Biotechnology Institute Co. Ltd
    • Marine Biosciences Kamaishi Research LaboratoryKitasato University
  • Hiroaki Kasai
    • Marine Biotechnology Institute Co. Ltd
    • Marine Biosciences Kamaishi Research LaboratoryKitasato University
Original Paper

DOI: 10.1007/s10482-011-9626-6

Cite this article as:
Yoon, J., Oku, N., Park, S. et al. Antonie van Leeuwenhoek (2012) 101: 133. doi:10.1007/s10482-011-9626-6

Abstract

A Gram-negative, strictly aerobic, reddish-pink pigmented, non-motile, rod-shaped strain designated N5DB8-4T, was isolated from an orange-striped sea anemone Diadumene lineata by a differential growth screening method. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Flammeovirgaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (89.1%) to Porifericola rhodea N5EA6-3A2BT. The strain could be differentiated phenotypically from recognized members of the family Flammeovirgaceae. The G+C content of the DNA is 52.6 mol%, the major respiratory quinone is menaquinone 7 (MK-7) and iso-C15:0, C16:1ω5c and iso-C15:1 G (the double-bond position indicated by capital letter is unknown) were the major fatty acids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain represents a novel taxon for which the name Tunicatimonas pelagia gen. nov., sp. nov. is proposed. The type strain of Tunicatimonas pelagia is N5DB8-4T (=KCTC 23473= NBRC 107804T).

Keywords

Tunicatimonas pelagia gen. nov., sp. nov.Concanavalin ADifferential growth screening method16S rRNA genePolyphasic taxonomy

Copyright information

© Springer Science+Business Media B.V. 2011