Antonie van Leeuwenhoek

, Volume 97, Issue 2, pp 189–200

Transferability of a tetracycline resistance gene from probiotic Lactobacillus reuteri to bacteria in the gastrointestinal tract of humans

  • Maria Egervärn
  • Hans Lindmark
  • Johan Olsson
  • Stefan Roos
Original Paper

DOI: 10.1007/s10482-009-9401-0

Cite this article as:
Egervärn, M., Lindmark, H., Olsson, J. et al. Antonie van Leeuwenhoek (2010) 97: 189. doi:10.1007/s10482-009-9401-0

Abstract

The potential of Lactobacillus reuteri as a donor of antibiotic resistance genes in the human gut was investigated by studying the transferability of the tetracycline resistance gene tet(W) to faecal enterococci, bifidobacteria and lactobacilli. In a double-blind clinical study, seven subjects consumed L. reuteri ATCC 55730 harbouring a plasmid-encoded tet(W) gene (tet(W)-reuteri) and an equal number of subjects consumed L. reuteri DSM 17938 derived from the ATCC 55730 strain by the removal of two plasmids, one of which contained the tet(W) gene. Faecal samples were collected before, during and after ingestion of 5 × 108 CFU of L.reuteri per day for 14 days. Both L. reuteri strains were detectable at similar levels in faeces after 14 days of intake but neither was detected after a two-week wash-out period. After enrichment and isolation of tetracycline resistant enterococci, bifidobacteria and lactobacilli from each faecal sample, DNA was extracted and analysed for presence of tet(W)-reuteri using a real-time PCR allelic discrimination method developed in this study. No tet(W)-reuteri signal was produced from any of the DNA samples and thus gene transfer to enterococci, bifidobacteria and lactobacilli during intestinal passage of the probiotic strain was non-detectable under the conditions tested, although transfer at low frequencies or to the remaining faecal bacterial population cannot be excluded.

Keywords

Allelic discriminationGene transferabilityHuman gutLactobacillus reuteriTetracycline resistancetet(W) gene

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Maria Egervärn
    • 1
    • 2
  • Hans Lindmark
    • 1
  • Johan Olsson
    • 3
  • Stefan Roos
    • 2
  1. 1.Microbiology DivisionNational Food AdministrationUppsalaSweden
  2. 2.Department of MicrobiologySwedish University of Agricultural SciencesUppsalaSweden
  3. 3.Good Food PracticeUppsalaSweden