Original Paper

Antonie van Leeuwenhoek

, 96:441

First online:

Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

  • Chai-Hoon KhooAffiliated withDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia
  • , Yoke-Kqueen CheahAffiliated withDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia Email author 
  • , Learn-Han LeeAffiliated withDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia
  • , Jiun-Horng SimAffiliated withDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia
  • , Noorzaleha Awang SallehAffiliated withDepartment of Chemistry Malaysia, Section of Biotechnology, Unit Microbiology
  • , Shiran Mohd SidikAffiliated withDepartment of Pathology, Faculty of Medicine and Health Sciences, University Putra Malaysia
  • , Son RaduAffiliated withDepartment of Food Science, Faculty of Food Science and Technology, University Putra Malaysia
  • , Sabrina SukardiAffiliated withDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia

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Abstract

The increased occurrence of Salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. This study is aimed to detect the prevalence of twenty different virulence factors among Salmonella enterica strains isolated from poultry and local indigenous vegetables in Malaysia via an optimized, rapid and specific multiplex PCR assay. The assay encompasses a total of 19 Salmonella pathogenicity islands genes and a quorum sensing gene (sdiA) in three multiplex reaction sets. A total of 114 Salmonella enterica isolates belonging to 38 different serovars were tested. Each isolate in under this study was found to possess up to 70% of the virulence genes tested and exhibited variable pathogenicity gene patterns. Reproducibility of the multiplex PCR assay was found to be 100% and the detection limit of the optimized multiplex PCR was tested with lowest detectable concentration of DNA 0.8 pg μl−1. This study demonstrated various Salmonella pathogenicity island virulence gene patterns even within the same serovar. This sets of multiplex PCR system provide a fast and reliable typing approach based on Salmonella pathogenicity islands, thus enabling an effective monitoring of emerging pathogenic Salmonella strains as an additional tool in Salmonella surveillance studies.

Keywords

Virulotyping Salmonella enterica Salmonella pathogenicity islands (SPIs) Multiplex PCR