Antonie van Leeuwenhoek

, Volume 96, Issue 3, pp 355–359

A database of microsatellite genotypes for Saccharomyces cerevisiae

  • Keith D. Richards
  • Matthew R. Goddard
  • Richard C. Gardner
Short Communication

DOI: 10.1007/s10482-009-9346-3

Cite this article as:
Richards, K.D., Goddard, M.R. & Gardner, R.C. Antonie van Leeuwenhoek (2009) 96: 355. doi:10.1007/s10482-009-9346-3

Abstract

A system for genotyping Saccharomyces cerevisiae is described based on a multiplex of ten microsatellite loci and the MAT locus. A database of genotypes has been developed for 246 yeast strains, including a large set of commercial wine yeasts, as well as 35 sequenced natural isolates currently being sequenced. The latter allow us, for the first time, to make direct comparisons of the relationship between DNA sequence data and microsatellite-based genotypes. The genotyping system provides a rapid and valuable system for strain identification as well as studying population genetics of S. cerevisiae.

Keywords

Saccharomyces cerevisiaeMicrosatelliteDNA fingerprintGenotyping

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Keith D. Richards
    • 1
  • Matthew R. Goddard
    • 1
  • Richard C. Gardner
    • 1
  1. 1.School of Biological SciencesUniversity of AucklandAucklandNew Zealand