Antonie van Leeuwenhoek

, 96:183

The ABC-transporter AtmA is involved in nickel and cobalt resistance of Cupriavidus metallidurans strain CH34

Original Paper

DOI: 10.1007/s10482-008-9303-6

Cite this article as:
Mikolay, A. & Nies, D.H. Antonie van Leeuwenhoek (2009) 96: 183. doi:10.1007/s10482-008-9303-6


Cupriavidus metallidurans CH34 genome contains an ortholog of Atm1p named AtmA (Rmet_0391, YP_582546). In Saccharomyces cerevisiae, the ABC-type transport system Atm1p is involved in export of iron–sulfur clusters from mitochondria into the cytoplasm for assembly of cytoplasmic iron–sulfur containing proteins. An ∆atmA mutant of C. metallidurans was sensitive to nickel and cobalt but not iron cations. AtmA increased also resistance to these cations in Escherichia coli strains that carry deletions of the genes for other nickel and cobalt transport systems. In C. metallidurans, atmA expression was not significantly induced by nickel and cobalt, but repressed by zinc. AtmA was purified as a 70 kDa protein after expression in E. coli. ATPase activity of AtmA was stimulated by nickel and cobalt.


NickelCobaltIron–sulfur centersABC-transport systemsAtmA

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Life Science Faculty, Institute for BiologyMartin-Luther-University Halle-WittenbergHalleGermany