Antonie van Leeuwenhoek

, Volume 95, Issue 1, pp 33–45

Discrimination among Bacillus thuringiensis H serotypes, serovars and strains based on 16S rRNA, gyrB and aroE gene sequence analyses

Original Paper

DOI: 10.1007/s10482-008-9285-4

Cite this article as:
Soufiane, B. & Côté, JC. Antonie van Leeuwenhoek (2009) 95: 33. doi:10.1007/s10482-008-9285-4

Abstract

Our aim was to investigate the capability of each of three genes, 16S rRNA, gyrB and aroE, to discriminate, first, among Bacillus thuringiensis H serotypes; second, among B. thuringiensis serovars from the same H serotype; and third, among B. thuringiensis strains from the same serovar. The 16S rRNA, gyrB and aroE genes were amplified from 21 B. thuringiensis H serotypes and their nucleotide sequences determined. Additional strains from four B. cereus sensu lato species were included for comparison purposes. These sequences were pair-wise compared and phylogenetic relationships were revealed. Each of the three genes under study could discriminate among B. thuringiensis H serotypes. The gyrB and aroE genes showed a discriminatory power among B. thuringiensis H serotypes up to nine fold greater than that of the 16S rRNA gene. The gyrB gene was retained for subsequent analyses to discriminate B. thuringiensis serovars from the same H serotype and to discriminate strains from same serovar. A total of 42 B. thuringiensis strains, which encompassed 25 serovars from 12 H serotypes, were analyzed. The gyrB gene nucleotide sequences were different enough as to be sufficient to discriminate among B. thuringiensis serovars from the same H serotype and among B. thuringiensis strains from the same serovar.

Keywords

16S rRNA gene aroE gene Bacillus thuringiensis Discriminatory power gyrB gene H serotypes Serovars 

Supplementary material

10482_2008_9285_MOESM1_ESM.doc (574 kb)
(DOC 575 kb)

Copyright information

© Her Majesty the Queen in Right of Canada 2008

Authors and Affiliations

  1. 1.Agriculture and Agri-Food CanadaResearch CentreSaint-Jean-sur-RichelieuCanada

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