Antonie van Leeuwenhoek

, 96:141

Biostructural analysis of the metal-sensor domain of CnrX from Cupriavidus metallidurans CH34

  • Guillaume Pompidor
  • Eric Girard
  • Antoine Maillard
  • Stéphanie Ramella-Pairin
  • Beate Bersch
  • Richard Kahn
  • Jacques Covès
Original Paper

DOI: 10.1007/s10482-008-9283-6

Cite this article as:
Pompidor, G., Girard, E., Maillard, A. et al. Antonie van Leeuwenhoek (2009) 96: 141. doi:10.1007/s10482-008-9283-6

Abstract

In Cupriavidus metallidurans CH34, the proteins CnrX, CnrY, and CnrH regulate the expression of the cnrCBA operon that codes for a cation-efflux pump involved in cobalt and nickel resistance. The periplasmic part of CnrX can be defined as the metal sensor in the signal transduction complex composed of the membrane-bound anti-sigma factor CnrY and the extra-cytoplasmic function sigma factor CnrH. A soluble form of CnrX was overproduced and purified. This protein behaves as a dimer in solution as judged from gel filtration, sedimentation velocity experiments, and NMR. Native crystals diffracting to 2.3 Å using synchrotron radiation were obtained using the hanging-drop vapor-diffusion method. They belong to the primitive monoclinic space group P21, with unit cell parameters a = 31.87, b = 74.80, c = 93.67 Å, β = 90.107°. NMR data and secondary structure prediction suggest that this protein is essentially formed by helices.

Keywords

CnrXCupriavidus metallidurans CH34Extra-cytoplamic-functionHeavy metalSignal transductionStructure

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Guillaume Pompidor
    • 1
  • Eric Girard
    • 1
  • Antoine Maillard
    • 1
  • Stéphanie Ramella-Pairin
    • 1
  • Beate Bersch
    • 2
  • Richard Kahn
    • 1
  • Jacques Covès
    • 1
  1. 1.Laboratoire des Protéines MembranairesInstitut de Biologie Structurale—Jean-Pierre Ebel, UMR 5075 CNRS-CEA-UJFGrenoble CedexFrance
  2. 2.Laboratoire de Résonance Magnétique NucléaireInstitut de Biologie Structurale—Jean-Pierre Ebel, UMR 5075 CNRS-CEA-UJFGrenoble CedexFrance