Antonie van Leeuwenhoek

, 94:377

Analysis of Salmonella Agona and Salmonella Weltevreden in Malaysia by PCR fingerprinting and antibiotic resistance profiling

  • Lee Learn-Han
  • Cheah Yoke-Kqueen
  • Noorzaleha Awang Salleh
  • Sabrina Sukardi
  • Sim Jiun-Horng
  • Khoo Chai-Hoon
  • Son Radu
Original Paper

DOI: 10.1007/s10482-008-9254-y

Cite this article as:
Learn-Han, L., Yoke-Kqueen, C., Salleh, N.A. et al. Antonie van Leeuwenhoek (2008) 94: 377. doi:10.1007/s10482-008-9254-y

Abstract

Forty-eight strains of Salmonella enterica subsp. enterica serovar Agona and 33 strains of Salmonella enterica subsp. enterica serovar Weltevreden were characterized by random amplified polymorphic DNA (RAPD) fingerprinting using 3 different arbitrary primer, Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR) and antimicrobial susceptibility testing. By using RAPD, 81 strains (44 strains of S. Agona and 33 strains of S. Weltevreden) can be clustered into 14 groups and 6 single isolates whereas ERIC-PCR produced 7 clusters and 3 single isolates. Thirteen antimicrobial agents were used and all the isolates were resistant to erythromycin and showed Multiple Antimicrobial Resistance indexes, ranging from 0.08 to 0.62. Poultry still remain as the common reservoir for multi-drug-resistant Salmonella. On the other hand, vegetables contaminated with S. Weltevreden showed a gain in antimicrobial resistance. Besides that, consistent antibiograms were observed from S. Weltevreden isolated at Kajang wet market on 2000/08/02.

Keywords

Salmonella AgonaSalmonella WeltevredenERIC-PCRRAPDMultiple antimicrobial resistanceComposite analysis

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Lee Learn-Han
    • 1
  • Cheah Yoke-Kqueen
    • 1
  • Noorzaleha Awang Salleh
    • 2
  • Sabrina Sukardi
    • 1
  • Sim Jiun-Horng
    • 1
  • Khoo Chai-Hoon
    • 1
  • Son Radu
    • 3
  1. 1.Department of Biomedical Science, Faculty of Medicine and Health SciencesUniversity Putra MalaysiaUPM SerdangMalaysia
  2. 2.Unit MicrobiologyDepartment of Chemistry MalaysiaPetaling JayaMalaysia
  3. 3.Department of Food Science, Faculty of Food Science and TechnologyUniversity Putra MalaysiaUPM SerdangMalaysia