Antonie van Leeuwenhoek

, Volume 90, Issue 1, pp 93–108

The production of a new extracellular putative long-chain saturated polyester by smooth variants of Mycobacterium vaccae interferes with Th1-cytokine production

  • Elisabeth Rodríguez-Güell
  • Gemma Agustí
  • Mercè Corominas
  • Pere-Joan Cardona
  • Isidre Casals
  • Teodor Parella
  • Marco-Antonio Sempere
  • Marina Luquin
  • Esther Julián
Article

DOI: 10.1007/s10482-006-9062-1

Cite this article as:
Rodríguez-Güell, E., Agustí, G., Corominas, M. et al. Antonie Van Leeuwenhoek (2006) 90: 93. doi:10.1007/s10482-006-9062-1

Abstract

Mycobacterium vaccae is of major pharmaceutical interest as an immunotherapeutic agent. Although M. vaccae 15483 ATCCT strain displays smooth and rough colonial morphologies on solid culture media, it is not known in which conditions M. vaccae switches from one colonial morphotype to the other or whether there are biological differences, especially immunological, between them. We have found that the change from a smooth to rough stable variant occurs spontaneously at 30 °C. The analysis of the composition of the cell wall in both variants showed that the smooth morphotype presents an extracellular material that has never previously been described and was identified as a long-chain saturated polyester that, interestingly, is not produced by the rough morphotype. Our results also indicate that this compound could be implicated in the spreading ability of smooth colonies. Proliferation, IFN-\(\upgamma\) and IL-12(p40) production by splenocyte cultures was significantly higher in mice immunised with the rough variant compared with those immunised with the smooth one. This latter finding suggests that the different colonial morphology of M. vaccae may affect the immunomodulatory effects induced from M. vaccae preparations.

Keywords

Colony morphologyExopolymerIFN-\(\upgamma\)Long-chain saturated polyesterMycobacterium vaccae

Abbreviations

ELISA

enzyme-linked immunosorbent assay

GC-MS

gas chromatography-mass spectrometry

HKR

heat-killed rough

HKS

heat-killed smooth

HPLC

high performance liquid chromatography

IR

infrared

LEDBP

bipolar-gradient LED

NMR

nuclear magnetic resonance

PFGSE

pulsed field-gradient spin-echo

RC

red compound

SEM

scanning electron microscopy

TLC

thin layer chromatography

TTA

2,3,5-triphenyl tetrazole

Supplementary material

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Elisabeth Rodríguez-Güell
    • 1
  • Gemma Agustí
    • 1
  • Mercè Corominas
    • 2
  • Pere-Joan Cardona
    • 3
  • Isidre Casals
    • 4
  • Teodor Parella
    • 5
  • Marco-Antonio Sempere
    • 6
  • Marina Luquin
    • 1
  • Esther Julián
    • 1
  1. 1.Departament de Genètica i de Microbiologia, Facultat de CiènciesUniversitat Autònoma de BarcelonaBellaterraSpain
  2. 2.Servei d’Immunologia-Al·lèrgiaHospital Universitari de Bellvitge L’Hospitalet de LlobregatSpain
  3. 3.Unitat de Tuberculosi ExperimentalFundació Institut per a la Investigació en Ciències de la Salut Germans Trias i PujolBadalonaSpain
  4. 4.Serveis CientificotècnicsUniversitat de BarcelonaBarcelonaSpain
  5. 5.Servei de Ressonància Magnètica NuclearUniversitat Autònoma de BarcelonaBellaterraSpain
  6. 6.Hospital Costa del SolMarbellaSpain