Antonie van Leeuwenhoek

, Volume 89, Issue 3, pp 359–365

Resuscitation-promoting factors: distribution among actinobacteria, synthesis during life-cycle and biological activity

Article

DOI: 10.1007/s10482-005-9039-5

Cite this article as:
Schroeckh, V. & Martin, K. Antonie Van Leeuwenhoek (2006) 89: 359. doi:10.1007/s10482-005-9039-5

Abstract

We have identified new members of the resuscitation-promoting factor (Rpf) protein family in numerous as yet unsequenced actinobacteria suggesting that Rpfs are common in this bacterial group. Formation and final delivery of the Rpf proteins varied from strain to strain and were dependent on growth conditions. Rpfs were found to act complementary to resuscitate other actinobacteria and might therefore be useful tools for the isolation of new actinobacterial species, especially from soil samples in which lots of microorganisms may have adopted dormant states.

Key words

ActinomycetesNon-culturable stateResuscitation-promoting factor

Abbreviations

Rpf

resuscitation-promoting factor

LMM

lactate minimal medium

GMM

galactose minimal medium

SMM

disodiumsuccinate minimal medium

TMHMM

transmembrane hidden Markov model (prediction of transmembrane helices in proteins)

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Leibniz Institute for Natural Products Research and Infection BiologyHans Knoell Institute (HKI)JenaGermany