Antonie van Leeuwenhoek

, Volume 87, Issue 1, pp 11–18

Diversity of cultivable actinobacteria in geographically widespread marine sediments

  • Luis A. Maldonado
  • James  E.M. Stach
  • Wasu Pathom-aree
  • Alan  C. Ward
  • Alan  T. Bull
  • Michael Goodfellow
Article

DOI: 10.1007/s10482-004-6525-0

Cite this article as:
Maldonado, L.A., Stach, J. ., Pathom-aree, W. et al. Antonie Van Leeuwenhoek (2005) 87: 11. doi:10.1007/s10482-004-6525-0

Abstract

Reports describing actinobacteria isolated from marine environments have been dominated by Micromonospora, Rhodococcus and Streptomyces species. Recent culture-independent studies have shown that marine environments contain a high diversity of actinobacterial species that are rarely, if at all, recovered by cultivation-based methods. In this study, it is shown that cultivation-independent methods can be used to guide the application of selective isolation methods. The detection of marine-derived actinobacterial species that have previously only been reported from terrestrial habitats is highlighted. This study provides good evidence that the previously described low diversity of actinobacterial species isolated from marine environments does not reflect an actual low species diversity, and that the use of informed selective isolation procedures can aid in the isolation of members of novel taxa.

Keywords

16S rRNA geneMarine environmentPCRActinobacteriaSelective isolationSequencing

Copyright information

© Springer 2005

Authors and Affiliations

  • Luis A. Maldonado
    • 1
    • 2
  • James  E.M. Stach
    • 3
  • Wasu Pathom-aree
    • 1
  • Alan  C. Ward
    • 1
  • Alan  T. Bull
    • 3
  • Michael Goodfellow
    • 1
  1. 1.School of BiologyUniversity of NewcastleNewcastle upon TyneUK
  2. 2.Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ)GuadalajaraMéxico
  3. 3.Research School of BiosciencesUniversity of KentCanterburyUK