Angiogenesis

, Volume 9, Issue 2, pp 83–91

Sulforaphane induces inhibition of human umbilical vein endothelial cells proliferation by apoptosis

  • Masahiro Asakage
  • Nelson H. Tsuno
  • Joji Kitayama
  • Takeshi Tsuchiya
  • Satomi Yoneyama
  • Jun Yamada
  • Yurai Okaji
  • Shoichi Kaisaki
  • Takuya Osada
  • Koki Takahashi
  • Hirokazu Nagawa
Original Paper

DOI: 10.1007/s10456-006-9034-0

Cite this article as:
Asakage, M., Tsuno, N.H., Kitayama, J. et al. Angiogenesis (2006) 9: 83. doi:10.1007/s10456-006-9034-0

Abstract

Sulforaphane (SUL), one of the isothiocyanates (ITCs), has recently been focused due to its inhibitory effects on tumor cell growth in vitro and in vivo, which is dependent on the direct effect on cancer cells. In the present study, we aimed to investigate the potential anti-angiogenic effect of SUL and its mechanism of action. Using the human umbilical vein endothelial cells (HUVECs) as a model of angiogenesis, we investigated the effect of SUL on the various steps of angiogenesis, including the proliferation of endothelial cells, tubular formation, and matrix metalloproteinase (MMP) production. Sulforaphane induced a dose-dependent decrease in the proliferative activity of endothelial cells, which was dependent on cell apoptosis. Also SUL inhibited tube formation on matrigel, but did not affect MMP production. The present results demonstrate the anti-angiogenic activity of SUL and its potential use as an anti-cancer drug is suggested.

Keywords

Angiogenesis inhibitorsApoptosisCell proliferationEndothelial cellsSulforaphane

Abbreviations

aFGF

Acidic fibroblast growth factor

DMSO

Dimethyl sulfoxide

ECs

Endothelial cells

FCS

Fetal calf serum

HUVECs

Human umbilical vein endothelial cells

ITCs

Isothiocyanates

MMP

Matrix metalloproteinase

PBS

Phosphate-buffered saline

SUL

Sulforaphane

TUNEL

Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Masahiro Asakage
    • 1
  • Nelson H. Tsuno
    • 1
    • 2
  • Joji Kitayama
    • 1
  • Takeshi Tsuchiya
    • 1
  • Satomi Yoneyama
    • 1
  • Jun Yamada
    • 1
  • Yurai Okaji
    • 2
  • Shoichi Kaisaki
    • 1
  • Takuya Osada
    • 1
  • Koki Takahashi
    • 2
  • Hirokazu Nagawa
    • 1
  1. 1.Department of Surgical OncologyUniversity of TokyoTokyoJapan
  2. 2.Faculty of Medicine, Department of Transfusion MedicineUniversity of TokyoTokyoJapan