Angiogenesis

, Volume 8, Issue 3, pp 205–216

Elucidation of the mechanisms underlying the angiogenic effects of ginsenoside Rg1in vivo and in vitro

  • Patrick Y.K. Yue
  • Daisy Y.L. Wong
  • W.Y. Ha
  • M.C. Fung
  • N.K. Mak
  • H.W. Yeung
  • H.W. Leung
  • Kelvin Chan
  • L. Liu
  • T.P.D. Fan
  • Ricky N.S. Wong
Article

DOI: 10.1007/s10456-005-9000-2

Cite this article as:
Yue, P.Y., Wong, D.Y., Ha, W. et al. Angiogenesis (2005) 8: 205. doi:10.1007/s10456-005-9000-2

Abstract

The major active constituents of ginseng are ginsenosides, and Rg1 is a predominant compound of the total extract. Recent studies have demonstrated that Rg1 can promote angiogenesis in vivo and in vitro. In this study, we used a DNA microarray technology to elucidate the mechanisms of action of Rg1. We report that Rg1 induces the proliferation of HUVECs, monitored using [3H]-thymidine incorporation and Trypan blue exclusion assays. Furthermore, Rg1 (150–600  nM) also showed an enhanced tube forming inducing effect on the HUVEC. Rg1 was also demonstrated to promote angiogenesis in an in vivo Matrigel plug assay, and increase endothelial sprouting in the ex vivo rat aorta ring assay. Differential gene expression profile of HUVEC following treatment with Rg1 revealed the expression of genes related to cell adhesion, migration and cytoskeleton, including RhoA, RhoB, IQGAP1, CALM2, Vav2 and LAMA4. Our results suggest that Rg1 can promote angiogenesis in multiple models, and this effect is partly due to the modulation of genes that are involved in the cytoskeletal dynamics, cell–cell adhesion and migration.

Keywords

angiogenesisgene expression profilingginsenosidesHUVECmicroarrayRg1

Supplementary material

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Patrick Y.K. Yue
    • 1
  • Daisy Y.L. Wong
    • 1
  • W.Y. Ha
    • 1
  • M.C. Fung
    • 2
  • N.K. Mak
    • 3
  • H.W. Yeung
    • 1
  • H.W. Leung
    • 1
  • Kelvin Chan
    • 1
  • L. Liu
    • 1
  • T.P.D. Fan
    • 4
  • Ricky N.S. Wong
    • 1
    • 3
  1. 1.Hung Lai Ching Laboratory of Biomedical Science, Research and Development Division, School of Chinese MedicineHong Kong Baptist UniversityKowloonHong Kong
  2. 2.Department of Biology, Science FacultyChinese University of Hong KongShatinHong Kong
  3. 3.Department of BiologyHong Kong Baptist UniversityKowloon TongHong Kong
  4. 4.Angiogenesis & TCM Laboratory, Department of PharmacologyUniversity of CambridgeCambridgeUK