Aerobiologia

, Volume 29, Issue 2, pp 315–319

Detection and source identification of airborne extended-spectrum beta-lactamase-producing Escherichia coli isolates in a chicken house

  • Song Li
  • Miaoqing Zhao
  • Yanling Li
  • Ling Zhang
  • Xianzhong Zhang
  • Zengmin Miao
Brief Communication

DOI: 10.1007/s10453-012-9273-z

Cite this article as:
Li, S., Zhao, M., Li, Y. et al. Aerobiologia (2013) 29: 315. doi:10.1007/s10453-012-9273-z

Abstract

To identify airborne dissemination of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) in a chicken house, airborne E. coli was collected from indoor air of a chicken house using six-stage Anderson sampler, and E. coli from chicken fecal samples were also isolated simultaneously. ESBL-producing E. coli isolates from indoor air and fecal samples were screened by a phenotypic confirmatory test according to CLSI recommendations. And then, the enterobacterial repetitive intergenic consensus polymerase chain reaction was performed to analyze the source of airborne ESBL-producing E. coli. The results showed that the ESBL-positive rates of E. coli isolates from feces and the indoor air were 56 % (18/32) and 40 % (6/15), respectively. Furthermore, airborne ESBL-producing E. coli isolates in the chicken house had 100 % genetic similarities with the strains from chicken feces, indicating that ESBL-producing E. coli from chicken feces could be aerosolized and spread to the air.

Keywords

Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli)Chicken houseEnterobacterial repetitive intergenic consensus (ERIC)Genetic similarity

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Song Li
    • 1
  • Miaoqing Zhao
    • 2
  • Yanling Li
    • 3
  • Ling Zhang
    • 3
  • Xianzhong Zhang
    • 3
  • Zengmin Miao
    • 3
  1. 1.College of Basic MedicineTaishan Medical UniversityTai’anChina
  2. 2.Pathology DepartmentShandong Provincial HospitalJinanChina
  3. 3.College of Life SciencesTaishan Medical UniversityTai’anChina