Annals of Biomedical Engineering

, Volume 42, Issue 9, pp 1978–1988

Autophagy Regulates Vascular Endothelial Cell eNOS and ET-1 Expression Induced by Laminar Shear Stress in an Ex Vivo Perfused System

  • Fengxia Guo
  • Xiaohong Li
  • Juan Peng
  • Yaling Tang
  • Qin Yang
  • Lushan Liu
  • Zuo Wang
  • Zhisheng Jiang
  • Ming Xiao
  • Chuyu Ni
  • Ruixing Chen
  • Dangheng Wei
  • Gui-xue Wang
Article

DOI: 10.1007/s10439-014-1033-5

Cite this article as:
Guo, F., Li, X., Peng, J. et al. Ann Biomed Eng (2014) 42: 1978. doi:10.1007/s10439-014-1033-5

Abstract

Vascular endothelial cell function responds to steady laminar shear stress; however, the underlying mechanisms are not fully elucidated. In the present study, we examined the effect of steady laminar shear stress on vascular endothelial cell autophagy and endothelial cell nitric oxide synthase (eNOS) and endothelin-1 (ET-1) expression using an ex vivo perfusion system. Human vascular endothelial cells and common arteries of New Zealand rabbits were pretreated with or without rapamycin or 3-MA for 30 min. These were then placed in an ex vivo cell perfusion system or an ex vivo organ perfusion system under static conditions (0 dynes/cm2) or steady laminar shear stress (5 or 15 dynes/cm2) for 1 h. In both ex vivo perfusion vascular endothelial cells and vascular vessel segment, steady laminar shear stress promoted autophagy and eNOS expression and inhibited ET-1 expression. Compared with steady laminar shear stress treatment alone, the pretreatment of autophagy inducer rapamycin obviously strengthened the expression of eNOS and decreased the expression of ET-1 in both the 5 and 15 dynes/cm2 treatment groups. Moreover, when pretreated with the autophagy inhibitor 3-MA, the eNOS expression was obviously inhibited and the ET-1 expression was reversed. These findings demonstrate that autophagy is upregulated under steady laminar shear stress, improving endothelial cell maintenance of vascular tone function.

Keywords

Autophagy Steady laminar shear stress Endothelial cells Endothelial nitric oxide synthase Endothelin-1 

Copyright information

© Biomedical Engineering Society 2014

Authors and Affiliations

  • Fengxia Guo
    • 1
    • 3
  • Xiaohong Li
    • 1
  • Juan Peng
    • 1
  • Yaling Tang
    • 1
  • Qin Yang
    • 1
  • Lushan Liu
    • 1
  • Zuo Wang
    • 1
  • Zhisheng Jiang
    • 1
  • Ming Xiao
    • 1
  • Chuyu Ni
    • 1
  • Ruixing Chen
    • 1
  • Dangheng Wei
    • 1
    • 2
  • Gui-xue Wang
    • 2
  1. 1.Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan ProvinceHengyangChina
  2. 2.Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of EducationBioengineering College of Chongqing UniversityChongqingChina
  3. 3.The Second People Hospital of Heze CityHezeChina