Annals of Biomedical Engineering

, Volume 42, Issue 9, pp 1889–1900

Comparison of Chondroitin Sulfate and Hyaluronic Acid Doped Conductive Polypyrrole Films for Adipose Stem Cells

  • Miina Björninen
  • Aliisa Siljander
  • Jani Pelto
  • Jari Hyttinen
  • Minna Kellomäki
  • Susanna Miettinen
  • Riitta Seppänen
  • Suvi Haimi
Article

DOI: 10.1007/s10439-014-1023-7

Cite this article as:
Björninen, M., Siljander, A., Pelto, J. et al. Ann Biomed Eng (2014) 42: 1889. doi:10.1007/s10439-014-1023-7

Abstract

Polypyrrole (PPy) is a conductive polymer that has aroused interest due to its biocompatibility with several cell types and high tailorability as an electroconductive scaffold coating. This study compares the effect of hyaluronic acid (HA) and chondroitin sulfate (CS) doped PPy films on human adipose stem cells (hASCs) under electrical stimulation. The PPy films were synthetized electrochemically. The surface morphology of PPy–HA and PPy–CS was characterized by an atomic force microscope. A pulsed biphasic electric current (BEC) was applied via PPy films non-stimulated samples acting as controls. Viability, attachment, proliferation and osteogenic differentiation of hASCs were evaluated by live/dead staining, DNA content, Alkaline phosphatase activity and mineralization assays. Human ASCs grew as a homogenous cell sheet on PPy–CS surfaces, whereas on PPy–HA cells clustered into small spherical structures. PPy–CS supported hASC proliferation significantly better than PPy–HA at the 7 day time point. Both substrates equally triggered early osteogenic differentiation of hASCs, although mineralization was significantly induced on PPy–CS compared to PPy–HA under BEC. These differences may be due to different surface morphologies originating from the CS and HA dopants. Our results suggest that PPy–CS in particular is a potential osteogenic scaffold coating for bone tissue engineering.

Keywords

Mesenchymal stem cellsOsteogenicElectrical stimulationPolysaccharide

Abbreviations

BEC

Biphasic electric current

ES

Electrical stimulation

hASC

Human adipose stem cells

PPy–CS

Chondroitin sulfate doped polypyrrole

PPy–HA

Hyaluronic acid doped polypyrrole

PS

Polystyrene cell culture plate

Copyright information

© Biomedical Engineering Society 2014

Authors and Affiliations

  • Miina Björninen
    • 1
    • 2
    • 3
  • Aliisa Siljander
    • 1
    • 2
    • 3
  • Jani Pelto
    • 4
  • Jari Hyttinen
    • 3
    • 5
  • Minna Kellomäki
    • 3
    • 5
  • Susanna Miettinen
    • 1
    • 2
    • 3
  • Riitta Seppänen
    • 1
    • 3
    • 5
    • 6
  • Suvi Haimi
    • 1
    • 2
    • 3
    • 7
  1. 1.Adult Stem Cells, Institute of Biomedical TechnologyUniversity of TampereTampereFinland
  2. 2.Science CentrePirkanmaa Hospital DistrictTampereFinland
  3. 3.BioMediTechTampereFinland
  4. 4.VTT Technical Research Centre of FinlandTampereFinland
  5. 5.Department of Biomedical Engineering, Department of Electronics and Communications EngineeringTampere University of TechnologyTampereFinland
  6. 6.Department of Eye, Ear, and Oral DiseasesTampere University HospitalTampereFinland
  7. 7.Department of Biomaterials Science and TechnologyUniversity of TwenteEnschedeThe Netherlands