Date: 18 Oct 2012
LDL Decreases the Membrane Compliance and Cell Adhesion of Endothelial Cells Under Fluid Shear Stress
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Atherosclerosis is an inflammatory disease of large and medium sized arteriole walls that is precipitated by elevated levels of low-density lipoprotein (LDL) cholesterol in the blood. However, the mechanisms that lead to the initiation of atherosclerosis are not fully understood. In this study, endothelial cells (ECs) were incubated with LDL for 24 h, and then the lipid was detected with Oil Red O staining and cholesterol ester was assayed with high-performance liquid chromatography (HPLC). F-actin was examined by fluorescence microscopy and the viscoelasticity of ECs was investigated using the micropipette aspiration technique. Then, a parallel-plate flow chamber device was used to observe the adhesion and retention of ECs under shear stress. The results demonstrated that elevated LDL significantly increased the cellular lipid content and induced the rearrangement of cytoskeletal F-actin. The initial rapid deformability (l/K 1 + l/K 2) was reduced by elevated cellular LDL levels, while membrane viscosity (μ) was increased by LDL accumulation. After treatment with 150 mg L−1 LDL for 24 h, the adhesion of ECs under fluid shear stress was significantly decreased (p < 0.05). These results suggested that LDL induced cellular lipid accumulation and cytoskeleton reorganization which increased the cellular stiffness and decreased the adhesion of ECs.
Associate Editor Konstantinos Konstantopoulos oversaw the review of this article.
Cicha, I., M. Goppelt-Struebe, A. Yilmaz, W. G. Daniel, and C. D. Garlichs. Endothelial dysfunction and monocyte recruitment in cells exposed to non-uniform shear stress. Clin. Hemorheol. Microcirc. 39:113–119, 2008.PubMed
Enomoto, M., H. Adachi, Y. Hirai, A. Fukami, A. Satoh, M. Otsuka, S. Kumagae, Y. Nanjo, K. Yoshikawa, E. Esaki, E. Kumagai, K. Ogata, A. Kasahara, E. Tsukagawa, K. Yokoi, K. Ohbu-Murayama, and T. Imaizumi. LDL-C/HDL-C ratio predicts carotid intima-media thickness progression better than HDL-C or LDL-C Alone. J. Lipids 2011:549137, 2011.PubMed
Lee, K., G. M. Saidel, and Penn, MS. Macromolecular transport in the arterial wall: alternative models for estimating barriers. Ann. Biomed. Eng. 33::1491–1503, 2005.CrossRef
Lowry, O., N. Rosebrough, A. Farr, and R. Randall. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275, 1951.PubMed
Ma, X., Y. W. Hu, Z. C. Mo, X. X. Li, X. H. Liu, J. Xiao, W. D. Yin, D. F. Liao, and C. K. Tang. NO-1886 up-regulates Niemann-Pick C1 protein (NPC1) expression through liver X receptor alpha signaling pathway in THP-1 macrophage-derived foam cells. Cardiovasc. Drugs Ther. 23:199–206, 2009.PubMedCrossRef
Padro, T., E. Pena, M. Garcia-Arguinzonis, V. Llorente-Cortes, and L. Badimón. Low-density lipoproteins impair migration of human coronary vascular smooth muscle cells and induce changes in the proteomic profile of myosin light chain. Cardiovasc. Res. 77:11–20, 2008.
Pourati, J., A. Maniotis, D. Spiegel, J. L. Schaffer, J. P. Butler, J. J. Fredberg, D. E. Ingber, D. Stamenovic, and N. Wang. Is cytoskeletal tension a major determinant of cell deformability in adherent endothelial cells? Am. J. Physiol. 274:C1283–C1289, 1998.PubMed
Shi, J., J. Wang, H. Zheng, W. Ling, J. Joseph, D. Li, J. L. Mehta, J. L. Mehta, U. Ponnappan, P. Lin, L. M. Fink, and M. Hauer-Jensen. Statins increase thrombomodulin expression and function in human endothelial cells by a nitric oxide-dependent mechanism and counteract tumor necrosis factor alpha-induced thrombomodulin downregulation. Blood Coagul. Fibrinolysis 14:575–585, 2003.PubMedCrossRef
Song, S., M. Kim, and J. H. Shin. Upstream mechanotaxis behavior of endothelial cells. Conf. Proc. IEEE Eng. Med. Biol. Soc. 2009:2106–2110, 2009.PubMed
Song, G., J. Qin, C. Yao, and Y. Ju. Effect of steep pulsed electric field on proliferation, viscoelasticity and adhesion of human hepatoma SMMC-7721 cells. Anticancer Res. 28:2245–2251, 2008.PubMed
Sung, K. L. P., L. A. Sung, M. Crimmins, S. J. Burakoff, and S. Chien. Dynamic changes in viscoelastic properties in cytotoxic T-lymphocyte-mediated killing. J. Cell Sci. 91:79–89, 1988.
Vincent, P. E., S. J. Sherwin, and P. D. Weinberg. The effect of a spatially heterogeneous transmural water flux on concentration polarization of low density lipoprotein in arteries. Biophysics 96:3102–3115, 2009.CrossRef
Wei, D. H., G. X. Wang, C. J. Tang, L. Q. Ye, L. Yang, L. H. Deng, L. S. Liu, Z. Wang, and C. K. Tang. Concentration polarization of low density lipoprotein at the distal end of carotid stenosis promotes atherogenesis. Acta Physiol. Sin. 59:831–839, 2007.
Xiao, L., G. Wang, T. Jiang, C. Tang, X. Wu, and T. Sun. Effects of shear stress on the number and function of endothelial progenitor cells adhered to specific matrices. J. Appl. Biomater. Biomech. 9:193–198, 2011.PubMed
- LDL Decreases the Membrane Compliance and Cell Adhesion of Endothelial Cells Under Fluid Shear Stress
Annals of Biomedical Engineering
Volume 41, Issue 3 , pp 611-618
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- 1. Key Laboratory for Arteriosclerology of Hunan Province, The Institute of Cardiovascular Disease, University of South China, Hengyang, 421001, China
- 2. Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400030, China