Annals of Biomedical Engineering

, Volume 40, Issue 2, pp 263–276

Multimodal 3D Imaging of Cells and Tissue, Bridging the Gap Between Clinical and Research Microscopy

  • Qin Miao
  • Anthony P. Reeves
  • Florence W. Patten
  • Eric J. Seibel
Article

DOI: 10.1007/s10439-011-0411-5

Cite this article as:
Miao, Q., Reeves, A.P., Patten, F.W. et al. Ann Biomed Eng (2012) 40: 263. doi:10.1007/s10439-011-0411-5

Abstract

Absorption dyes are widely used in traditional cytology and pathology clinical practice, while fluorophores and nanoparticles are more often used in biologic research. Optical projection tomographic microscopy (OPTM) is a platform technology that can image the same specimen in multiple modes in 3D, providing morphologic and molecular information concurrently and in exact co-registration. The depth-of-field of a high numerical aperture objective is extended by scanning the focal plane through the sample to generate an optical projection image. Samples of cells or tissue are brought into the OPTM instrument through a microcapillary tube filled with optical index-matching gel. Multiple optical projection images are taken from different perspectives by rotating the tube. Computed tomography (CT) algorithms are applied to these optical projection images to reconstruct 3D structure of the sample. Image segmentation and analysis based on these 3D images provide quantitative biosignatures for cancer diagnosis that represents a clear improvement over conventional 2D image analysis. In this article, we introduce the OPTM platform, optical Cell-CT, and Tissue-CT instruments, and some applications using these OPTM instruments.

Keywords

Multimodal imagingAbsorption stainFluorescenceNanoparticlesImage segmentation3D visualizationCancer diagnosisNuclear-to-cytoplasmic ratio

Copyright information

© Biomedical Engineering Society 2011

Authors and Affiliations

  • Qin Miao
    • 1
  • Anthony P. Reeves
    • 2
  • Florence W. Patten
    • 3
    • 4
  • Eric J. Seibel
    • 4
  1. 1.Department of BioengineeringUniversity of WashingtonSeattleUSA
  2. 2.Department of Electrical and Computer EngineeringCornell UniversityIthacaUSA
  3. 3.VisionGate Inc.PhoenixUSA
  4. 4.Department of Mechanical EngineeringUniversity of WashingtonSeattleUSA