Mechanical, Compositional, and Structural Properties of the Post-natal Mouse Achilles Tendon
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During post-natal development, tendons undergo a well orchestrated process whereby extensive structural and compositional changes occur in synchrony to produce a normal tissue. Conversely, during the repair response to injury, structural and compositional changes occur, but in this case, a mechanically inferior tendon is produced. As a result, the process of development has been postulated as a potential paradigm through which improved adult tissue healing may occur. In this study we measured the mechanical, compositional, and structural properties in the post-natal mouse Achilles tendon at 4, 7, 10, 14, 21, and 28 days old. Throughout post-natal development, the mechanical properties, collagen content, fibril diameter mean, and fibril diameter standard deviation increased. Biglycan expression decreased and decorin expression and fiber organization were unchanged. This study provides a new mouse model that can be used to quantitatively examine mechanical development, as well as compositional and structural changes and biological mechanisms, during post-natal tendon development. This model is advantageous due to the large number of genetically modified mice and commercially available assays that are not available in other animal models. A mouse model therefore allows future mechanistic studies to build on this work.
- Ansorge, H. L., X. Meng, G. Zhang, G. Veit, M. Sun, J. F. Klement, D. P. Beason, L. J. Soslowsky, M. Koch, and D. E. Birk. Type XIV collagen regulates fibrillogenesis: premature collagen fibril growth and tissue dysfunction in null mice. J. Biol. Chem. 284:8427–8438, 2009. CrossRef
- Beredjiklian, P. K. Biologic aspects of flexor tendon laceration and repair. J. Bone Joint Surg. Am. 85-A:539–550, 2003.
- Birk, D. E., R. A. Hahn, C. Y. Linsenmayer, and E. I. Zycband. Characterization of collagen fibril segments from chicken embryo cornea, dermis and tendon. Matrix Biol. 15:111–118, 1996. CrossRef
- Birk, D. E., and R. Mayne. Localization of collagen types I, III and V during tendon development. Changes in collagen types I and III are correlated with changes in fibril diameter. Eur. J. Cell Biol. 72:352–361, 1997.
- Birk, D. E., E. I. Zycband, D. A. Winkelmann, and R. L. Trelstad. Collagen fibrillogenesis in situ: fibril segments are intermediates in matrix assembly. Proc. Natl Acad. Sci. USA 86:4549–4553, 1989. CrossRef
- Bland, Y. S., and D. E. Ashhurst. Fetal and postnatal development of the patella, patellar tendon and suprapatella in the rabbit; changes in the distribution of the fibrillar collagens. J. Anat. 190(Pt 3):327–342, 1997. CrossRef
- Booth, F. W., and C. M. Tipton. Ligamentous strength measurements in pre-pubescent and pubescent rats. Growth 34:177–185, 1970.
- Boykiw, R., P. Sciore, C. Reno, L. Marchuk, C. B. Frank, and D. A. Hart. Altered levels of extracellular matrix molecule mRNA in healing rabbit ligaments. Matrix Biol. 17:371–378, 1998. CrossRef
- Danielson, K. G., H. Baribault, D. F. Holmes, H. Graham, K. E. Kadler, and R. V. Iozzo. Targeted disruption of decorin leads to abnormal collagen fibril morphology and skin fragility. J. Cell Biol. 136:729–743, 1997. CrossRef
- Derwin, K. A., and L. J. Soslowsky. A quantitative investigation of structure–function relationships in a tendon fascicle model. J. Biomech. Eng. 121:598–604, 1999. CrossRef
- Derwin, K. A., L. J. Soslowsky, W. D. Green, and S. H. Elder. A new optical system for the determination of deformations and strains: calibration characteristics and experimental results. J. Biomech. 27:1277–1285, 1994. CrossRef
- Ehrlich, H. P., P. A. Lambert, G. C. Saggers, R. L. Myers, and R. M. Hauck. Dynamic changes appearing in collagen fibers during intrinsic tendon repair. Ann. Plast. Surg. 54:201–206, 2005. CrossRef
- Ezura, Y., S. Chakravarti, A. Oldberg, I. Chervoneva, and D. E. Birk. Differential expression of lumican and fibromodulin regulate collagen fibrillogenesis in developing mouse tendons. J. Cell Biol. 151:779–788, 2000. CrossRef
- Festing, M. F. Design and statistical methods in studies using animal models of development. ILAR J. 47:5–14, 2006.
- Franchi, M., M. Fini, M. Quaranta, V. De Pasquale, M. Raspanti, G. Giavaresi, V. Ottani, and A. Ruggeri. Crimp morphology in relaxed and stretched rat Achilles tendon. J. Anat. 210:1–7, 2007. CrossRef
- Gimbel, J. A., J. P. Van Kleunen, S. Mehta, S. M. Perry, G. R. Williams, and L. J. Soslowsky. Supraspinatus tendon organizational and mechanical properties in a chronic rotator cuff tear animal model. J. Biomech. 37:739–749, 2004. CrossRef
- Lin, T. W., L. Cardenas, and L. J. Soslowsky. Tendon properties in interleukin-4 and interleukin-6 knockout mice. J. Biomech. 38:99–105, 2005.
- McBride, D. J., R. L. Trelstad, and F. H. Silver. Structural and mechanical assessment of developing chick tendon. Int. J. Biol. Macromol. 10:194–200, 1988. CrossRef
- Mikic, B., E. Amadei, K. Rossmeier, and L. Bierwert. Sex matters in the establishment of murine tendon composition and material properties during growth. J. Orthop. Res. 28:631–638, 2010.
- Moore, M. J., and A. De Beaux. A quantitative ultrastructural study of rat tendon from birth to maturity. J. Anat. 153:163–169, 1987.
- Neuman, R. E., and M. A. Logan. The determination of hydroxyproline. J. Biol. Chem. 184:299–306, 1950.
- Oryan, A., and A. H. Shoushtari. Histology and ultrastructure of the developing superficial digital flexor tendon in rabbits. Anat. Histol. Embryol. 37:134–140, 2008. CrossRef
- Parry, D. A., G. R. Barnes, and A. S. Craig. A comparison of the size distribution of collagen fibrils in connective tissues as a function of age and a possible relation between fibril size distribution and mechanical properties. Proc. R. Soc. Lond. B Biol. Sci. 203:305–321, 1978. CrossRef
- Parry, D. A., A. S. Craig, and G. R. Barnes. Tendon and ligament from the horse: an ultrastructural study of collagen fibrils and elastic fibres as a function of age. Proc. R. Soc. Lond. B Biol. Sci. 203:293–303, 1978. CrossRef
- Peltz, C. D., S. M. Perry, C. L. Getz, and L. J. Soslowsky. Mechanical properties of the long-head of the biceps tendon are altered in the presence of rotator cuff tears in a rat model. J. Orthop. Res. 27:416–420, 2009. CrossRef
- Rigozzi, S., R. Muller, and J. G. Snedeker. Collagen fibril morphology and mechanical properties of the Achilles tendon in two inbred mouse strains. J. Anat. 216:724–731, 2010. CrossRef
- Thomopoulos, S., G. Hattersley, V. Rosen, M. Mertens, L. Galatz, G. R. Williams, and L. J. Soslowsky. The localized expression of extracellular matrix components in healing tendon insertion sites: an in situ hybridization study. J. Orthop. Res. 20:454–463, 2002. CrossRef
- Thomopoulos, S., G. R. Williams, J. A. Gimbel, M. Favata, and L. J. Soslowsky. Variation of biomechanical, structural, and compositional properties along the tendon to bone insertion site. J. Orthop. Res. 21:413–419, 2003. CrossRef
- Webster, T. Putting a strain on workers’ health. Compens. Work. Cond. Spring:29–31, 1999. http://www.bls.gov/opub/cwc/archive/spring1999brief2.pdf.
- Woo, S. L., R. E. Debski, J. Zeminski, S. D. Abramowitch, S. S. Saw, and J. A. Fenwick. Injury and repair of ligaments and tendons. Annu. Rev. Biomed. Eng. 2:83–118, 2000. CrossRef
- Woo, S. L., R. H. Gelberman, N. G. Cobb, D. Amiel, K. Lothringer, and W. H. Akeson. The importance of controlled passive mobilization on flexor tendon healing. A biomechanical study. Acta Orthop. Scand. 52:615–622, 1981. CrossRef
- Woo, S. L., C. A. Orlando, M. A. Gomez, C. B. Frank, and W. H. Akeson. Tensile properties of the medial collateral ligament as a function of age. J. Orthop. Res. 4:133–141, 1986. CrossRef
- Zhang, G., S. Chen, S. Goldoni, B. W. Calder, H. C. Simpson, R. T. Owens, D. J. McQuillan, M. F. Young, R. V. Iozzo, and D. E. Birk. Genetic evidence for the coordinated regulation of collagen fibrillogenesis in the cornea by decorin and biglycan. J. Biol. Chem. 284:8888–8897, 2009. CrossRef
- Zhang, G., Y. Ezura, I. Chervoneva, P. S. Robinson, D. P. Beason, E. T. Carine, L. J. Soslowsky, R. V. Iozzo, and D. E. Birk. Decorin regulates assembly of collagen fibrils and acquisition of biomechanical properties during tendon development. J. Cell Biochem. 98:1436–1449, 2006. CrossRef
- Zhang, G., B. B. Young, Y. Ezura, M. Favata, L. J. Soslowsky, S. Chakravarti, and D. E. Birk. Development of tendon structure and function: regulation of collagen fibrillogenesis. J. Musculoskelet. Neuronal Interact. 5:5–21, 2005.
- Mechanical, Compositional, and Structural Properties of the Post-natal Mouse Achilles Tendon
Annals of Biomedical Engineering
Volume 39, Issue 7 , pp 1904-1913
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- 1. McKay Orthopaedic Research Laboratory, University of Pennsylvania, 424 Stemmler Hall, 3450 Hamilton Walk, Philadelphia, PA, 19104-6081, USA
- 2. Department of Pathology and Cell Biology, College of Medicine, University of South Florida, Tampa, FL, 33612, USA