Effects of Hydroxyapatite and Biostite® on Osteogenic Induction of hMSC
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When isolated from the iliac crest human mesenchymal stem cells (hMSC) differentiate into osteoblast-like cells with appropriate stimulation in culture. This in vitro study tested the hypothesis that Biostite® and hydroxyapatite (HA) affect proliferation and differentiation of hMSC into osteoblastic cells. Cell proliferation was determined by measuring 3H-thymidine incorporation into DNA and typical markers of osteoblastic phenotype were determined by RT-PCR assay. No differences emerged in cell proliferation cultures with Biostite® or hydroxyapatite (HA), but gene expression analysis revealed higher expression of collagen, alkaline phosphatase (ALP), osteopontin and bone sialoprotein (BSP) in the presence of Biostite®. TGFβ2 production, as assessed by an Elisa kit, and Runx2 expression by RT-PCR, were greater in Biostite cultures, suggesting Biostite® provides a better environment for hMSC differentiation into osteoblasts and is, potentially, a more promising bone-filling material than HA.
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- Effects of Hydroxyapatite and Biostite® on Osteogenic Induction of hMSC
Annals of Biomedical Engineering
Volume 38, Issue 3 , pp 640-648
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- Osteoblastic differentiation
- Extracellular matrix production
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- Author Affiliations
- 1. Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy
- 2. Department of Dermatology and Plastic Surgery, University La Sapienza, Rome, Italy
- 3. Piazza B. Buozzi 36, Ospedalicchio, Perugia, Italy
- 4. Forensic Medicine Institute, University of Perugia, Perugia, Italy
- 5. via Beethoven 1, Ellera di Corciano, Perugia, Italy