Annals of Biomedical Engineering

, Volume 37, Issue 7, pp 1322–1330

Evaluation of Human Endothelial Cells Post Stent Deployment in a Cardiovascular Simulator In Vitro

  • Marie A. Punchard
  • Eoin D. O’Cearbhaill
  • Joseph N. Mackle
  • Peter E. McHugh
  • Terry J. Smith
  • Catherine Stenson-Cox
  • Valerie Barron
Article

DOI: 10.1007/s10439-009-9701-6

Cite this article as:
Punchard, M.A., O’Cearbhaill, E.D., Mackle, J.N. et al. Ann Biomed Eng (2009) 37: 1322. doi:10.1007/s10439-009-9701-6

Abstract

Percutaneous stent implantation has revolutionized the clinical treatment of occluded arteries. Nevertheless, there is still a large unmet need to prevent re-occlusion after implantation. Consequently, a niche exists for a cost-effective pre-clinical method of evaluating novel interventional devices in human models. Therefore, the development of a coronary model artery offers tremendous potential for the treatment of endothelial cell dysfunction and restenosis. As a first step, we employ tissue-engineering principles to examine the effect of stent deployment upon endothelial cells in a tubular in vitro system capable of replicating the coronary artery biomechanical environment. In particular, the cellular and molecular changes pertaining to inflammation, proliferation, and death were assessed after stent deployment. Real-time quantitative PCR demonstrated increased expression of genes encoding for E-Selectin, ICAM-1, and VCAM-1; markers associated with an inflammatory response in vivo. Further, an increase in the pro-apoptotic protein Bax was paralleled with a decrease in the anti-apoptotic protein Bcl-2; however, apoptotic morphology was not observed. Interestingly, transcription of c-fos increased, whereas Ki67 levels fell over the same period. One hypothesis is that these results are in response to the altered local hemodynamic environment induced by stent deployment. Most significantly, this study highlights the potential of a biomimetic hemodynamic bioreactor combined with a gene expression analysis to evaluate, with greater specificity, the performance and interaction of stents with the endothelial layer in a controlled environment.

Keywords

Stent Pre-clinical evaluation Model human artery Stent deployment in vitro Cellular response 

Copyright information

© Biomedical Engineering Society 2009

Authors and Affiliations

  • Marie A. Punchard
    • 1
  • Eoin D. O’Cearbhaill
    • 1
    • 2
  • Joseph N. Mackle
    • 1
    • 3
  • Peter E. McHugh
    • 1
    • 2
  • Terry J. Smith
    • 1
  • Catherine Stenson-Cox
    • 1
  • Valerie Barron
    • 1
    • 3
  1. 1.National Centre for Biomedical Engineering, Orbsen BuildingNational University of Ireland, GalwayGalwayIreland
  2. 2.Department of Mechanical and Biomedical EngineeringNational University of Ireland, GalwayGalwayIreland
  3. 3.Regenerative Medicine Institute (REMEDI), National Centre for Biomedical Engineering Science (NCBES), Orbsen BuildingNational University of Ireland, GalwayGalwayIreland