Annals of Biomedical Engineering

, Volume 36, Issue 2, pp 185–194

Regulatory Effects of Mechanical Strain on the Chondrogenic Differentiation of MSCs in a Collagen-GAG Scaffold: Experimental and Computational Analysis

  • Louise A. McMahon
  • Alan J. Reid
  • Veronica A. Campbell
  • Patrick J. Prendergast
Article

DOI: 10.1007/s10439-007-9416-5

Cite this article as:
McMahon, L.A., Reid, A.J., Campbell, V.A. et al. Ann Biomed Eng (2008) 36: 185. doi:10.1007/s10439-007-9416-5

Abstract

The effective treatment of cartilage defects by tissue engineering requires an improved understanding of the effect of mechanical forces on cell differentiation within three-dimensional (3D) matrices. The objective of this study was to investigate the effects of mechanical constraint and cyclic tensile strain on the chondrogenic differentiation of mesenchymal stem cells (MSCs) in a 3D collagen type I-glycosaminoglycan (GAG) scaffold. A multi-station uniaxial stretching bioreactor was fabricated to facilitate application of cyclic strain to the constructs cultured in a chondrogenic medium. Mechanical constraint, created by uniaxial clamping, prevented the cell-mediated contraction of the scaffolds and resulted in a reduction in the rate of GAG synthesis as measured by [35S] sulfate incorporation relative to unconstrained controls. However, the rate of GAG synthesis was increased following application of continuous 10% cyclic tensile loading at 1 Hz for 7 days. A poroelastic finite element analysis of the 3D scaffold computed a maximum fluid flow of 19 μm/s and maximum principal strains of 8% under 10% stretch suggesting these magnitudes were sufficient to mechano-regulate the chondrogenic differentiation process.

Keywords

Chondrogenic differentiationMesenchymal stem cellsCollagen-GAG scaffoldMechanoregulationTensile strainComputational analysis

Copyright information

© Biomedical Engineering Society 2007

Authors and Affiliations

  • Louise A. McMahon
    • 1
  • Alan J. Reid
    • 2
  • Veronica A. Campbell
    • 1
    • 3
  • Patrick J. Prendergast
    • 1
    • 2
  1. 1.Trinity Centre for Bioengineering, School of EngineeringTrinity CollegeDublinIreland
  2. 2.Department of Mechanical Engineering, School of EngineeringTrinity CollegeDublinIreland
  3. 3.Department of Physiology, School of MedicineTrinity CollegeDublinIreland