Annals of Biomedical Engineering

, Volume 34, Issue 1, pp 3–14

Engineering Luminescent Quantum Dots for In Vivo Molecular and Cellular Imaging

Authors

  • Andrew M. Smith
    • Departments of Biomedical Engineering and ChemistryEmory University and Georgia Institute of Technology
  • Gang Ruan
    • Departments of Biomedical Engineering and ChemistryEmory University and Georgia Institute of Technology
  • Matthew N. Rhyner
    • Departments of Biomedical Engineering and ChemistryEmory University and Georgia Institute of Technology
    • Departments of Biomedical Engineering and ChemistryEmory University and Georgia Institute of Technology
    • Departments of Biomedical Engineering and ChemistryEmory University and Georgia Institute of Technology
Nanobioengineering

DOI: 10.1007/s10439-005-9000-9

Cite this article as:
Smith, A.M., Ruan, G., Rhyner, M.N. et al. Ann Biomed Eng (2006) 34: 3. doi:10.1007/s10439-005-9000-9

Semiconductor quantum dots are luminescent nanoparticles that are under intensive development for use as a new class of optical imaging contrast agents. Their novel properties such as optical tunability, improved photostability, and multicolor light emission have opened new opportunities for imaging living cells and in vivo animal models at unprecedented sensitivity and spatial resolution. Combined with biomolecular engineering strategies for tailoring the particle surfaces at the molecular level, bioconjugated quantum dot probes are well suited for imaging single-molecule dynamics in living cells, for monitoring protein–protein interactions within specific intracellular locations, and for detecting diseased sites and organs in deep tissue. In this article, we describe the engineering principles for preparing high-quality quantum dots and for conjugating the dots to biomolecular ligands. We also discuss recent advances in using quantum dots for in vivo molecular and cellular imaging.

Keywords

NanoparticlesNanotechnologyFluorescenceLiving cellsLiving animalsMolecular imagingCytotoxicityCationic peptidesBioconjugationDynamic light scattering

ABBREVIATIONS

FRET

fluorescence resonance energy transfer

PEG

polyethylene glycol

QD

quantum dot

RES

reticuloendothelial system

TOP

trioctylphosphine

TOPO

trioctylphosphine oxide

Copyright information

© Springer Science+Business Media, Inc. 2006