Nanobioengineering

Annals of Biomedical Engineering

, Volume 34, Issue 1, pp 3-14

First online:

Engineering Luminescent Quantum Dots for In Vivo Molecular and Cellular Imaging

  • Andrew M. SmithAffiliated withDepartments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology
  • , Gang RuanAffiliated withDepartments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology
  • , Matthew N. RhynerAffiliated withDepartments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology
  • , Shuming NieAffiliated withDepartments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of TechnologyDepartments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Semiconductor quantum dots are luminescent nanoparticles that are under intensive development for use as a new class of optical imaging contrast agents. Their novel properties such as optical tunability, improved photostability, and multicolor light emission have opened new opportunities for imaging living cells and in vivo animal models at unprecedented sensitivity and spatial resolution. Combined with biomolecular engineering strategies for tailoring the particle surfaces at the molecular level, bioconjugated quantum dot probes are well suited for imaging single-molecule dynamics in living cells, for monitoring protein–protein interactions within specific intracellular locations, and for detecting diseased sites and organs in deep tissue. In this article, we describe the engineering principles for preparing high-quality quantum dots and for conjugating the dots to biomolecular ligands. We also discuss recent advances in using quantum dots for in vivo molecular and cellular imaging.

Keywords

Nanoparticles Nanotechnology Fluorescence Living cells Living animals Molecular imaging Cytotoxicity Cationic peptides Bioconjugation Dynamic light scattering