Article

Annals of Biomedical Engineering

, Volume 33, Issue 11, pp 1469-1490

First online:

Mechanobiology in the Third Dimension

  • John A. PedersenAffiliated withBiomedical Engineering Department, Northwestern University
  • , Melody A. SwartzAffiliated withBiomedical Engineering Department, Northwestern UniversityIntegrative Biosciences Institute, École Polytechnique Fédérale de Lausanne (EPFL)Laboratory for Mechanobiology and Morphogenesis, Integrative Biosciences Institute, Swiss Federal Institute of Technology Lausanne (EPFL) Email author 

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Abstract

Cells are mechanically coupled to their extracellular environments, which play critical roles in both communicating the state of the mechanical environment to the cell as well as in mediating cellular response to a variety of stimuli. Along with the molecular composition and mechanical properties of the extracellular matrix (ECM), recent work has demonstrated the importance of dimensionality in cell-ECM associations for controlling the sensitive communication between cells and the ECM. Matrix forces are generally transmitted to cells differently when the cells are on two-dimensional (2D) vs. within three-dimensional (3D) matrices, and cells in 3D environments may experience mechanical signaling that is unique vis-à-vis cells in 2D environments, such as the recently described 3D-matrix adhesion assemblies. This review examines how the dimensionality of the extracellular environment can affect in vitro cell mechanobiology, focusing on collagen and fibrin systems.

Key Words

Cell mechanics Tissue mechanics Collagen Fibrin Tissue engineering Hydrogel Fibroblast Stress shielding Cell strain