Tissue Engineering of Human Heart Valve Leaflets: A Novel Bioreactor for a Strain-Based Conditioning Approach
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Current mechanical conditioning approaches for heart valve tissue engineering concentrate on mimicking the opening and closing behavior of the leaflets, either or not in combination with tissue straining. This study describes a novel approach by mimicking only the diastolic phase of the cardiac cycle, resulting in tissue straining. A novel, yet simplified, bioreactor system was developed for this purpose by applying a dynamic pressure difference over a closed tissue engineered valve, thereby inducing dynamic strains within the leaflets. Besides the use of dynamic strains, the developing leaflet tissues were exposed to prestrain induced by the use of a stented geometry. To demonstrate the feasibility of this strain-based conditioning approach, human heart valve leaflets were engineered and their mechanial behavior evaluated. The actual dynamic strain magnitude in the leaflets over time was estimated using numerical analyses. Preliminary results showed superior tissue formation and non-linear tissue-like mechanical properties in the strained valves when compared to non-loaded tissue strips. In conclusion, the strain-based conditioning approach, using both prestrain and dynamic strains, offers new possibilities for bioreactor design and optimization of tissue properties towards a tissue-engineered aortic human heart valve replacement.
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- Tissue Engineering of Human Heart Valve Leaflets: A Novel Bioreactor for a Strain-Based Conditioning Approach
Annals of Biomedical Engineering
Volume 33, Issue 12 , pp 1778-1788
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- Heart valve prostheses
- Mechanical conditioning
- Industry Sectors
- Author Affiliations
- 1. Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands
- 2. Clinic for Cardiovascular Surgery, University Hospital Zürich, Raemistrasse, Zürich, Switzerland
- 3. Department of Biomedical Engineering, Laboratory for Biomechanics and Tissue Engineering, Eindhoven University of Technology, P.O. Box 513, Building W-hoog 4.112, 5600, MB, Eindhoven, The Netherlands