Annals of Biomedical Engineering

, Volume 33, Issue 8, pp 1034–1041

Contactless Multiple Wavelength Photoplethysmographic Imaging: A First Step Toward “SpO2 Camera” Technology


    • Erasmus Medical Center, Biomedical Engineering
    • TNO Prevention and Health
  • F. Mastik
    • Erasmus Medical Center, Biomedical Engineering
  • A. F. W. van der Steen
    • Erasmus Medical Center, Biomedical Engineering
    • Interuniversity Cardiology Institute of the Netherlands (ICIN)

DOI: 10.1007/s10439-005-5763-2

Cite this article as:
Wieringa, F.P., Mastik, F. & Steen, A.F.W.v.d. Ann Biomed Eng (2005) 33: 1034. doi:10.1007/s10439-005-5763-2


We describe a route toward contactless imaging of arterial oxygen saturation (SpO2) distribution within tissue, based upon detection of a two-dimensional matrix of spatially resolved optical plethysmographic signals at different wavelengths. As a first step toward SpO2-imaging we built a monochrome CMOS-camera with apochromatic lens and 3λ-LED-ringlight (λ1 = 660 nm, λ2 = 810 nm, λ3 = 940 nm; 100 LEDs λ−1). We acquired movies at three wavelengths while simultaneously recording ECG and respiration for seven volunteers. We repeated this experiment for one volunteer at increased frame rate, additionally recording the pulse wave of a pulse oximeter. Movies were processed by dividing each image frame into discrete Regions of Interest (ROIs), averaging 10 × 10 raw pixels each. For each ROI, pulsatile variation over time was assigned to a matrix of ROI-pixel time traces with individual Fourier spectra. Photoplethysmograms correlated well with respiration reference traces at three wavelengths. Increased frame rates revealed weaker pulsations (main frequency components 0.95 and 1.9 Hz) superimposed upon respiration-correlated photoplethysmograms, which were heartbeat-related at three wavelengths. We acquired spatially resolved heartbeat-related photoplethysmograms at multiple wavelengths using a remote camera. This feasibility study shows potential for non-contact 2-D imaging reflection-mode pulse oximetry. Clinical devices, however, require further development.


Pulse oximetryMultispectral imagingContactless measurement of respiration and heart action

Copyright information

© Biomedical Engineering Society 2005