Microfluidics and Nanofluidics

, Volume 10, Issue 5, pp 941–964

Microfluidic whole-blood immunoassays

Review Paper

DOI: 10.1007/s10404-010-0718-9

Cite this article as:
Jiang, H., Weng, X. & Li, D. Microfluid Nanofluid (2011) 10: 941. doi:10.1007/s10404-010-0718-9

Abstract

Immunoassay is one of the most widely used biomedical diagnostic methods due to its sensitivity and specificity. Microfluidic lab-on-a-chip technology has the advantages of portability, integration, and automation. The combination of these two technologies leads to a pathway for point-of-care diagnostics using the unprocessed samples such as the whole blood. This article reviews the recent advancement and the major development in the microfluidic-based whole-blood immunoassays. After a survey of the recent studies on microfluidic whole-blood immunoassays, an in-depth review about the detection methods that can be miniaturized and integrated in the immunoassay chips is provided. Point-of-care diagnostics applications require developing a fully integrated, disposable, low-cost, and handheld microfluidic device for the whole-blood immunoassay. In this regard, some comments and suggestions for future research are given.

Keywords

MicrofluidicsImmunoassayWhole bloodOptical detectionNon-optical detectionLab-on-a-chip

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Department of Mechanical and Mechatronics EngineeringUniversity of WaterlooWaterlooCanada