Microfluidics and Nanofluidics

, Volume 8, Issue 5, pp 677–685

Microfluidic network-based combinatorial dilution device for high throughput screening and optimization

  • Kangsun Lee
  • Choong Kim
  • Geunhui Jung
  • Tae Song Kim
  • Ji Yoon Kang
  • Kwang W. Oh
Research Paper

DOI: 10.1007/s10404-009-0500-z

Cite this article as:
Lee, K., Kim, C., Jung, G. et al. Microfluid Nanofluid (2010) 8: 677. doi:10.1007/s10404-009-0500-z

Abstract

We present a combinatorial dilution device using a three-layer microfluidic network that can produce systematic variations of buffer and additive solutions in a combinatorial fashion for high throughput screening and optimization. A proof-of-concept device providing seven combinations (ABC/D, AB/D, BC/D, AC/D, A/D, B/D, and C/D) of three additive samples (A, B, and C) into a buffer solution (D) has been demonstrated. Such combinations are often used in simplex-centroid mixture DOE (design of experiments), useful techniques to minimize the experimental efforts at maximal information output with systematic variations of large-scale components. Based on mathematical and electrical modeling and computational fluid dynamic simulation, the device has been designed, fabricated, and characterized.

Keywords

Combinatorial device Microfluidic network High throughput screening Design of experiments 

Supplementary material

10404_2009_500_MOESM1_ESM.doc (452 kb)
Supplementary material 1 (DOC 451 kb)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Kangsun Lee
    • 1
  • Choong Kim
    • 2
  • Geunhui Jung
    • 2
  • Tae Song Kim
    • 2
  • Ji Yoon Kang
    • 2
  • Kwang W. Oh
    • 1
  1. 1.SMALL (Nanobio Sensors and MicroActuators Learning Laboratory), Department of Electrical EngineeringUniversity at Buffalo, The State University of New York (SUNY at Buffalo)BuffaloUSA
  2. 2.Nano-Bioresearch CenterKorea Institute of Science and Technology (KIST)SeoulKorea

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