Microfluidics and Nanofluidics

, Volume 1, Issue 4, pp 346–355

A microfluidic chip for heterogeneous immunoassay using electrokinetical control

  • Guoqing Hu
  • Yali Gao
  • Philip M. Sherman
  • Dongqing Li
Research Paper

DOI: 10.1007/s10404-005-0040-0

Cite this article as:
Hu, G., Gao, Y., Sherman, P.M. et al. Microfluid Nanofluid (2005) 1: 346. doi:10.1007/s10404-005-0040-0

Abstract

This article presents the development of a novel, automated, electrokinetically controlled heterogeneous immunoassay on a poly(dimethylsiloxane) (PDMS) microfluidic chip. A numerical method has been developed to simulate the electrokinetically driven, time-dependent delivery processes of reagents and washing solutions within the complex microchannel network. Based on the parameters determined from the numerical simulations, fully automated on-chip experiments to detect Helicobacter pylori were accomplished by sequentially changing the applied electric fields. Shortened assay time and much less reagent consumptions are achieved by using this microchannel chip while the detection limit is comparable to the conventional assay. There is a good agreement between the experimental result and numerical prediction, demonstrating the effectiveness of using CFD to assist the experimental studies of microfluidic immunoassay.

Keywords

Microfluidics Lab-on-a-chip Heterogeneous immunoassay Numerical simulation H. pylori Electroosmosis 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Guoqing Hu
    • 1
  • Yali Gao
    • 1
  • Philip M. Sherman
    • 2
  • Dongqing Li
    • 1
  1. 1.Department of Mechanical and Industrial EngineeringUniversity of TorontoTorontoCanada
  2. 2.Departments of Paediatrics and Laboratory Medicine & PathobiologyUniversity of TorontoTorontoCanada