Japanese Journal of Ophthalmology

, Volume 50, Issue 2, pp 103–110

Systemic T Cell Response to Toxoplasma gondii Antigen in Patients with Ocular Toxoplasmosis

  • Fatih Fatoohi
  • Grégoire Jacques Noël Cozon
  • Martine Wallon
  • Laurent Kodjikian
  • François Peyron
LABORATORY INVESTIGATION

DOI: 10.1007/s10384-005-0295-8

Cite this article as:
Fatoohi, F., Cozon, G., Wallon, M. et al. Jpn J Ophthalmol (2006) 50: 103. doi:10.1007/s10384-005-0295-8

Abstract

Purpose

Analysis of systemic cellular response to Toxoplasma antigen in patients with ocular toxoplasmosis.

Methods

Activated (CD25+) T cells were detected by flow cytometry after a 7-day culture of whole blood from patients with ocular (n = 16) or asymptomatic (n = 14) toxoplasmosis, and controls (n = 10), in the presence of soluble Toxoplasma antigen (ST-Ag). Interferon (IFN)-γ, interleukin (IL) 4, and IL-10 were measured in culture supernatants by enzyme-linked immunosorbent assay.

Results

Higher percentages of CD25+ T cells were detected in ST-Ag-activated cultures from Toxoplasma-infected patients, with or without ocular lesions (37.0 ± 19.1% or 41.1 ± 19.3%, respectively) than from controls (3.2 ± 1.2%) (P < 0.0001). Differences were not statistically significant between asymptomatic and ocular toxoplasmosis (P > 0.4) or among congenital, acquired, and undetermined ocular toxoplasmosis (P > 0.2). Higher levels of IFN-γ were detected in ST-Ag-stimulated blood cultures from infected patients than in those from controls (P < 0.0001), with no difference between patients with asymptomatic or ocular toxoplasmosis (P > 0.05). IL-10 was detected only in activated culture supernatants from three patients with ocular toxoplasmosis and two patients with asymptomatic toxoplasmosis. IL-4 was never produced in ST-Ag-activated cultures.

Conclusions

Systemic cellular response to ST-Ag does not differ between the patients with ocular and asymptomatic toxoplasmosis with regard to activation markers and type 1 cytokine production. Jpn J Ophthalmol 2006;50:103–110 © Japanese Ophthalmological Society 2006

Key words

cellular immunityinterferon-γinterleukin-4retinochoroiditisToxo-plasma gondii

Copyright information

© Japanese Ophthalmological Society 2006

Authors and Affiliations

  • Fatih Fatoohi
    • 1
    • 2
  • Grégoire Jacques Noël Cozon
    • 2
  • Martine Wallon
    • 1
    • 3
  • Laurent Kodjikian
    • 4
  • François Peyron
    • 1
    • 3
  1. 1.E. A. 3087 Parasitologie, Faculté de Médecine, Lyon NordUniversité Cl. Bernard Lyon1France
  2. 2.Laboratoire d'Immunologie, Hôpital de la Croix-RousseHospices Civils de LyonFrance
  3. 3.Service de Parasitologie, Hôpital de la Croix-RousseHospices Civils de LyonFrance
  4. 4.Service d'Ophtalmologie, Hôpital de la Croix-RousseHospices Civils de LyonFrance
  5. 5.Laboratoire d'Immunologie, Hôpital de la Croix-RousseHospices Civils de LyonLyonFrance