Journal of Pest Science

, Volume 86, Issue 1, pp 99–106

A survey of microbial antagonists of Agriotes wireworms from Germany and Italy

  • Regina G. Kleespies
  • Claudia Ritter
  • Gisbert Zimmermann
  • Frank Burghause
  • Simon Feiertag
  • Andreas Leclerque
Original Paper

DOI: 10.1007/s10340-012-0447-9

Cite this article as:
Kleespies, R.G., Ritter, C., Zimmermann, G. et al. J Pest Sci (2013) 86: 99. doi:10.1007/s10340-012-0447-9

Abstract

Wireworms of the genus Agriotes (Coleoptera: Elateridae) are severe and widespread agricultural pests affecting numerous crops throughout the world and causing damage of economic importance. Between 2008 and 2011, a survey of the occurrence of natural microbial antagonists of wireworms was conducted. Altogether, 3,420 wireworms and two dead beetles from agricultural habitats and grassland of various parts of Germany and 700 lab-reared wireworms from Italy were screened for the presence of disease. Of these, 283 selected dead and moribund Agriotes specimens were diagnosed by light and electron microscopy for insect pathogens; 66 wireworms contained unspecified bacterial infections; and 86 specimens displayed fungal growth with 25 infected by the entomopathogenic taxa Beauveria bassiana, Metarhizium anisopliae, Isaria fumosorosea, Isaria spp. and Lecanicillium sp. Nematodes were observed in 29 dead individuals, and 141 individuals showed no distinguishable pathogens. In one dead Agriotes larva, an intracellular bacterium was identified as a new Rickettsiella pathotype named ‘Rickettsiella agriotidis’. The survey resulted in a natural infection rate of 0.66 %. The possible use of these pathogens as biocontrol agents is discussed.

Keywords

WirewormClick beetleAgriotesElateridaeMetarhiziumBeauveriaIsariaRickettsiellaEntomopathogen

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Regina G. Kleespies
    • 1
  • Claudia Ritter
    • 2
  • Gisbert Zimmermann
    • 1
    • 3
  • Frank Burghause
    • 4
  • Simon Feiertag
    • 1
    • 2
  • Andreas Leclerque
    • 1
    • 5
  1. 1.Institute for Biological ControlJulius Kühn-Institut (JKI), Federal Research Centre for Cultivated PlantsDarmstadtGermany
  2. 2.Institute for Agriculture and Fishing Research, Mecklenburg-Vorpommern, Centre for Field Vegetable ProductionGülzowGermany
  3. 3.MühltalGermany
  4. 4.Dienstleistungszentrum Ländlicher Raum (DLR), Rheinhessen-Nahe-HunsrückBad KreuznachGermany
  5. 5.Institut für Mikrobiologie und GenetikTechnische Universität DarmstadtDarmstadtGermany