Chromatographia

, Volume 76, Issue 7, pp 355–362

Paramagnetic Particles Isolation of Influenza Oligonucleotide Labelled with CdS QDs

  • Ludmila Krejcova
  • David Hynek
  • Pavel Kopel
  • Vojtech Adam
  • Jaromir Hubalek
  • Libuse Trnkova
  • Rene Kizek
Original

DOI: 10.1007/s10337-012-2327-0

Cite this article as:
Krejcova, L., Hynek, D., Kopel, P. et al. Chromatographia (2013) 76: 355. doi:10.1007/s10337-012-2327-0

Abstract

In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine–adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 °C, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 μg mL−1. The detection limit (3 signal/noise) was estimated as 15 ng mL−1 of ODN-SH-Cd.

Keywords

BiosensorsVoltammetryAutomated separationNanoparticlesQuantum dotsHybridizationVirus

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Ludmila Krejcova
    • 1
  • David Hynek
    • 1
    • 2
  • Pavel Kopel
    • 1
    • 2
  • Vojtech Adam
    • 1
    • 2
  • Jaromir Hubalek
    • 2
    • 3
  • Libuse Trnkova
    • 1
    • 2
  • Rene Kizek
    • 1
    • 2
  1. 1.Department of Chemistry and Biochemistry, Faculty of AgronomyMendel University in BrnoBrnoCzech Republic
  2. 2.Central European Institute of Technology, Brno University of TechnologyBrnoCzech Republic
  3. 3.Department of Microelectronics, Faculty of Electrical Engineering and CommunicationBrno University of TechnologyBrnoCzech Republic