, Volume 73, Issue 1 Supplement, pp 137-142,
Open Access This content is freely available online to anyone, anywhere at any time.
Date: 08 Jan 2011

Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol


Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5–32% depending on polarities and concentrations. Results could be dramatically improved in terms of separation and quantification using RP-LC with a mercaptoethanol precolumn derivatization aimed at avoiding ITCs precipitation. The cancer chemoprotective allyl-ITC and sulforaphane were found in cabbage extracts at 1.2 and 2.7 μg g−1 fresh weight, respectively.

Presented at: 16th International Symposium on Separation Science, Recent Advancements in Chromatography and Capillary Electromigration Techniques. Rome, Italy, 6–10 September, 2010.